Helicase Structures:
BACH1 / DNAB / EIF4A / HCV NS3 / HPV E1 / MJDEAD / PCRA / RCK/P54 / RecG / RecQ / Rep / Rep40 / RepA / Rho / SecA / SV40 / T7 Gene 4 / UAP56 / UvrB / UvsW
         
Name Complex DNA/RNA
 PDBID Reference
         
BACH1 BRCA1

DNA
SF2

 1T15

Clapperton, J.A., Manke, I.A., Lowery, D.M., Ho, T., Haire, L.F., Yaffe, M.B.,Smerdon, S.J.:
Structure and mechanism of BRCA1 BRCT domain recognition of phosphorylated BACH1 with implications for cancer.
Nat. Struct. Mol. Biol. 11 pp. 512-8 (2004)
         
DNAB
N-terminus 		  DNA
SF3 		1B79

Fass, D., Bogden, C. E., Berger, J. M.:
Crystal Structure of the N-Terminal Domain of the Dnab Hexameric Helicase
Structure 7 pp. 691 (1999)
         
DNAB
N-terminus
(NMR) 		  DNA
SF3 		1JWE Weigelt, J., Brown, S.E., Miles, C.S., Dixon, N.E.,Otting, G.:
NMR structure of the N-terminal domain of E. coli DnaB helicase: implications for structure rearrangements in the helicase hexamer.
Structure 7 pp. 681-90 (1999)
         
EIF4A
C-terminus 		 

RNA
SF2

1FUK Caruthers, J. M., Johnson, E. R., Mckay, D. B.:
Crystal Structure of Yeast Initiation Factor 4A, a Dead-Box RNA Helicase
Proc.Nat.Acad.Sci.USA 97 pp. 13080 (2000)
         
EIF4A
complete
model 		  RNA
SF2 		1FUU Caruthers, J. M., Johnson, E. R., Mckay, D. B.:
Crystal Structure of Yeast Initiation Factor 4A, a Dead-Box RNA Helicase
Proc.Nat.Acad.Sci.USA 97 pp. 13080 (2000)
         
EIF4A
N-terminus 		  RNA
SF2 		1QDE Benz, J., Trachsel, H., Baumann, U.:
Crystal Structure of the ATPase Domain of Translation Initiation Factor 4A from Saccharomyces Cerevisiae-the Prototype of the Dead Box Protein Family
Structure 7 pp. 671 (1999)
         
EIF4A
N-terminus 		  RNA
SF2 		1QVA Johnson, E. R., Mckay, D. B.:
Yeast Initiation Factor 4A N-Terminal Domain
RNA 5 (1999)
         
HCV NS3 ssDNA RNA/DNA
SF2 		1A1V Kim, J. L., Morgenstern, K. A., Griffith, J. P., Dwyer, M. D., Thomson, J. A., Murcko, M. A., Lin, C., Caron, P. R.:
Hepatitis C virus NS3 RNA helicase domain with a bound oligonucleotide: the crystal structure provides insights into the mode of unwinding.
Structure 6 pp. 89 (1998)
         
HCV NS3
helicase and protease domains		   RNA/DNA
SF2 		1CU1

Yao, N., Reichert, P., Taremi, S.S., Prosise, W.W.,Weber, P.C.:
Molecular views of viral polyprotein processing revealed by the crystal structure of the hepatitis C virus bifunctional protease-helicase.
Structure Fold Des. 7 pp. 1353-63 (1999)
         
HCV NS3   RNA/DNA
SF2 		1HEI Yao, N., Hesson, T., Cable, M., Hong, Z., Kwong, A. D., Le, H. V., Weber, P. C.:
Structure of the hepatitis C virus RNA helicase domain.
Nat. Struct. Biol. 4 pp. 463 (1997)
         
HCV NS3
Domain 2 		  RNA/DNA
SF2 		1JR6 Liu, D., Wang, Y.S., Gesell, J.J.,Wyss, D.F.:
Solution structure and backbone dynamics of an engineered arginine-rich subdomain 2 of the hepatitis C virus NS3 RNA helicase.
J. Mol. Biol. 314 pp. 543-61 (2001)
         
HCV NS3
Domain 2 		  RNA/DNA
SF2 		1ONB

Liu, D., Wang, Y.S., Gesell, J.J.,Wyss, D.F.:
Solution structure and backbone dynamics of an engineered arginine-rich subdomain 2 of the hepatitis C virus NS3 RNA helicase.
J. Mol. Biol. 314 pp. 543-61 (2001)
         
HCV NS3   RNA/DNA
SF2 		8OHM Cho, H. S., Ha, N. C., Kang, L. W., Chung, K. M., Back, S. H., Jang, S. K., Oh, B. H.:
Crystal structure of RNA helicase from genotype 1b hepatitis C virus. A feasible mechanism of unwinding duplex RNA.
J. Biol. Chem. 273 pp. 15045 (1998)
         
HPV E1
DNA-binding domain		  

DNA
SF1

1F08 Enemark, E.J., Chen, G., Vaughn, D.E., Stenlund, A.,Joshua-Tor, L.:
Crystal structure of the DNA binding domain of the replication initiation protein E1 from papillomavirus.
Mol Cell 6 pp. 149-58 (2000)
         
HPV E1   DNA
SF1 		1KSX Enemark, E.J., Stenlund, A.,Joshua-Tor, L.:
Crystal structures of two intermediates in the assembly of the papillomavirus replication initiation complex.
EMBO J 21 pp. 1487-96 (2002)
         
HPV E1   DNA
SF1 		1KSY Enemark, E.J., Stenlund, A.,Joshua-Tor, L.:
Crystal structures of two intermediates in the assembly of the papillomavirus replication initiation complex.
EMBO J 21 pp. 1487-96 (2002)
         
HPV E1 E2 DNA
SF1 		1TUE Abbate, E.A., Berger, J.M.,Botchan, M.R.:
The X-ray structure of the papillomavirus helicase in complex with its molecular matchmaker E2.
Genes Dev 18 pp. 1981-96 (2004)
         
MJDEAD   RNA
SF2		 1HV8 Story, R. M., Li, H., Abelson, J. N.:
Crystal Structure of a Dead Box Protein from the Hyperthermophile Methanococcus Jannaschii Proc.Nat.Acad.Sci.USA 98 pp. 1465 (2001)
         
PCRA   DNA
SF1 		1PJR Subramanya, H. S., Bird, L. E., Brannigan, J. A., Wigley, D. B.:
Crystal structure of a DExx box DNA helicase.
Nature 384 pp. 379 (1996)
         
PCRA
mutant 		ADPNP DNA
SF1 		1QHG Soultanas, P., Dillingham, M. S., Velankar, S. S., Wigley, D.B.:
DNA Binding Mediates Conformational Changes and Metal Ion Coordination in the Active Site of Pcra Helicase
J.Mol.Biol. 290 pp. 137 (1999)
         
PCRA ADPNP DNA
SF1 		1QHH Soultanas, P., Dillingham, M. S., Velankar, S. S., Wigley, D.B.:
DNA Binding Mediates Conformational Changes and Metal Ion Coordination in the Active Site of Pcra Helicase
J.Mol.Biol. 290 pp. 137 (1999)
         
PCRA product DNA
SF1 		2PJR Velankar, S. S., Soultanas, P., Dillingham, M. S., Subramanya, H. S., Wigley, D. B.:
Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism.
Cell 97 pp. 75 (1999)
         
PCRA substrate DNA
SF1 		3PJR Velankar, S. S., Soultanas, P., Dillingham, M. S., Subramanya, H. S., Wigley, D. B.:
Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism.
Cell 97 pp. 75 (1999)
         
RCK/P54   RNA
SF2		 1VEC Matsui, T., Hogetsu, K., Akao, Y., Tanaka, M., Sato, T., Kumasaka, T.,Tanaka, N.:
Crystallization and X-ray analysis of the N-terminal core domain of a tumour-associated human DEAD-box RNA helicase, rck/p54.
Acta Crystallogr D Biol Crystallogr 60 pp. 156-9 (2004)
         
RecG 3-way DNA junction DNA
SF2		 1GM5 Singleton, M.R., Scaife, S., WIgley, D.B.:
Structural Analysis of DNA Replication Fork Reversal by RecG.
Cell 107, pp. 79 (2001)
         
RecQ
HRC-domain 		  DNA
SF2		 1D8B Liu, Z., Macias, M. J., Bottomley, M. J., Stier, G., Linge, J. P., Nilges, M., Bork, P., Sattler, M.:
The Three-Dimensional Structure of the Hrdc Domain and Implications for the Werner and Bloom Syndrome
Proteins Structure 7 pp. 1557 (1999)
         
RecQ   DNA
SF2		 1OYW Bernstein, D.A., Zittel, M.C.,Keck, J.L.:
High-resolution structure of the E.coli RecQ helicase catalytic core.
EMBO J 22 pp. 4910-21 (2003)
         
RecQ ATP-gS DNA
SF2		 1OYY Bernstein, D.A., Zittel, M.C.,Keck, J.L.:
High-resolution structure of the E.coli RecQ helicase catalytic core.
EMBO J 22 pp. 4910-21 (2003)
         
Rep ssDNA DNA
SF2		 1UAA Korolev, S., Hsieh, J., Gauss, G. H., Lohman, T. M., Waksman, G.:
Major domain swiveling revealed by the crystal structures of complexes of E. coli Rep helicase bound to single-stranded DNA and ADP.
Cell 90 pp. 635 (1997)
         
Rep40   DNA
SF3		 1S9H James, J.A., Escalante, C.R., Yoon-Robarts, M., Edwards, T.A., Linden, R.M.,Aggarwal, A.K.:
Crystal structure of the SF3 helicase from adeno-associated virus type 2.
Structure (Camb) 11 pp. 1025-35 (2003)
         
Rep40 ADP DNA
SF3		 1U0J  James, J. A., Aggarwal, A. K., Linden, R. M., Escalante, C. R.:
Structure of Adeno-Associated Virus Type 2 Rep40-Adp Complex: Insight Into Nucleotide Recognition and Catalysis by Superfamily 3 Helicases
Proc.Nat.Acad.Sci.USA 101 pp. 12455 (2004)
         
RepA   DNA
SF3		 1G8Y Niedenzu, T., Roeleke, D., Bains, G., Scherzinger, E., Saenger, W.:
Crystal Structure of the Hexameric Replicative Helicase Repa of Plasmid Rsf1010
J.Mol.Biol. 306 pp. 479 (2001)
         
RepA Sulfate DNA
SF3		 1NLF Xu, H., Strater, N., Schroeder, W., Bottcher, C., Ludwig, K., Saenger, W.:
Structure of DNA Helicase Repa in Complex with Sulfate at 1.95A Resolution Implicates Structural Changes to an `Open' Form
Acta Crystallogr., Sect.D 59 pp. 815 (2003)
         
RepA   DNA
SF3		 1OLO  Ziegelin, G., Niedenzu, T., Lurz, R., Saenger, W., Lanka, E.:
Hexameric Rsf1010 Helicase Repa: The Structural and Functional Importance of Single Amino Acid Residues
Nucleic Acids Res. 31 pp. 5917 (2003)
         
Rho   DNA/RNA
SF3		 1A62 Allison, T. J., Wood, T. C., Briercheck, D. M., Rastinejad, F.,Richardson, J. P., Rule, G. S.:
Crystal structure of the RNA-binding domain from transcription termination factor rho.
Nat. Struct. Biol. 5 pp. 352 (1998)
         
Rho
(NMR) 		  DNA/RNA
SF3		 1A63 Allison, T. J., Wood, T. C., Briercheck, D. M., Rastinejad, F.,Richardson, J. P., Rule, G. S.:
Crystal structure of the RNA-binding domain from transcription termination factor rho.
Nat. Struct. Biol. 5 pp. 352 (1998)
         
Rho   DNA/RNA
SF3		 1A8V Bogden, C. E., Fass, D., Bergman, N., Nichols, M. D., Berger, J. M.:
The structural basis for terminator recognition by the Rho transcription termination factor.
Mol. Cell. 3 pp. 487 (1999)
         
Rho ssDNA DNA/RNA
SF3		 1PV4 Xu, H., Strater, N., Schroeder, W., Bottcher, C., Ludwig, K., Saenger, W.:
Structure of DNA Helicase Repa in Complex with Sulfate at 1.95A Resolution Implicates Structural Changes to an `Open' Form
Acta Crystallogr., Sect.D 59 pp. 815 (2003)
         
Rho ssRNA, ADPNP DNA/RNA
SF3		 1PVO Skordalakes, E., Berger, J. M.:
Structure of the Rho Transcription Terminator: Mechanism of Mrna Recognition and Helicase Loading
Cell (Cambridge, Mass.) 114 pp. 135 (2003)
         
Rho RNA DNA/RNA
SF3		 2A8V Bogden, C. E., Fass, D., Bergman, N., Nichols, M. D., Berger, J. M.:
The structural basis for terminator recognition by the Rho transcription termination factor.
Mol Cell 3 pp. 487 (1999)
         
SecA (protein translocase)   SF2 1M6N Hunt, J. F., Weinkauf, S., Henry, L., Fak, J. J., Mcnicholas, P., Oliver, D. B., Deisenhofer, J.:
Nucleotide Control of Interdomain Interactions in the Conformational Reaction Cycle of Seca
Science 297 pp. 2018 (2002)
         
SecA (protein translocase) Mg-ADP SF2 1M74 Hunt, J. F., Weinkauf, S., Henry, L., Fak, J. J., Mcnicholas, P., Oliver, D. B., Deisenhofer, J.:
Nucleotide Control of Interdomain Interactions in the Conformational Reaction Cycle of Seca
Science 297 pp. 2018 (2002)
         
SecA (protein translocase)   SF2 1NKT Sharma, V., Arockiasamy, A., Ronning, D. R., Savva, C. G., Holzenburg, A., Braunstein, M., Jacobs Jr., W. R., Sacchettini, J. C.:
Crystal Structure of M. Tuberculosis Seca, a Preprotein Translocating ATPase
Proc.Nat.Acad.Sci.USA 100 pp. 2243 (2003)
         
SecA (protein translocase)   SF2 1NL3 Sharma, V., Arockiasamy, A., Ronning, D. R., Savva, C. G., Holzenburg, A., Braunstein, M., Jacobs Jr., W. R., Sacchettini, J. C.:
Crystal Structure of M. Tuberculosis Seca, a Preprotein Translocating ATPase
Proc.Nat.Acad.Sci.USA 100 pp. 2243 (2003)
         
SecA (protein translocase) ADP SF2 1TF2 Osborne, A. R., Clemons Jr., W. M., Rapoport, T. A.:
A Large Conformational Change of the Translocation ATPase Seca.
Proc.Nat.Acad.Sci.USA 101 pp. 10937 (2004)
         
SecA (protein translocase)   SF2 1TF5 Osborne, A. R., Clemons Jr., W. M., Rapoport, T. A.:
A Large Conformational Change of the Translocation ATPase Seca.
Proc.Nat.Acad.Sci.USA 101 pp. 10937 (2004)
         
SV40   DNA
SF2		 1N25 Li, D., Zhao, R., Lilyestrom, W., Gai, D., Zhang, R., Decaprio, J. A., Fanning, E., Jochimiak, A., Szakonyi, G., Chen, X. S.:
Structure of the Replicative Helicase of the Oncoprotein Sv40 Large Tumour Antigen
Nature 423 pp. 512 (2003)
         
T7 Gene 4 Ring helicase   DNA
SF3		 1CR0 Sawaya, M. R., Guo, S., Tabor, S., Richardson, C. C., Ellenberger, T.:
Crystal Structure of the Helicase Domain from the Replicative Helicase-Primase of Bacteriophage T7
Cell 99 pp. 167 (1999)
         
T7 Gene 4 Ring helicase DTTP DNA
SF3		 1CR1 Sawaya, M. R., Guo, S., Tabor, S., Richardson, C. C., Ellenberger, T.:
Crystal Structure of the Helicase Domain from the Replicative Helicase-Primase of Bacteriophage T7
Cell 99 pp. 167 (1999)
         
T7 Gene 4 Ring helicase DATP DNA
SF3		 1CR2 Sawaya, M. R., Guo, S., Tabor, S., Richardson, C. C., Ellenberger, T.:
Crystal Structure of the Helicase Domain from the Replicative Helicase-Primase of Bacteriophage T7
Cell 99 pp. 167 (1999)
         
T7 Gene 4 Ring helicase DTDP DNA
SF3		 1CR4 Sawaya, M. R., Guo, S., Tabor, S., Richardson, C. C., Ellenberger, T.:
Crystal Structure of the Helicase Domain from the Replicative Helicase-Primase of Bacteriophage T7
Cell 99 pp. 167 (1999)
         
T7 Gene 4 Ring helicase ANPNP DNA
SF3		 1E0J Singleton, M. R., Sawaya, M. R., Ellenberger, T., Wigley, D. B.:
Crystal Structure of T7 Gene 4 Ring Helicase Indicates a Mechanism for Sequential Hydrolysis of Nucleotides
Cell 101 pp. 589 (2000)
         
T7 Gene 4 Ring helicase   DNA
SF3		 1E0K Singleton, M. R., Sawaya, M. R., Ellenberger, T., Wigley, D. B.:
Crystal Structure of T7 Gene 4 Ring Helicase Indicates a Mechanism for Sequential Hydrolysis of Nucleotides
Cell 101 pp. 589 (2000)
         
T7 Gene 4 Ring helicase   DNA
SF3		 1NUI Kato, M., Ito, T., Wagner, G., Richardson, C. C., Ellenberger, T.:
Modular Architecture of the Bacteriophage T7 Primase Couples RNA Primer Synthesis to DNA Synthesis
Mol. Cell 11 pp. 1349 (2003)
         
T7 Gene 4 Ring helicase   DNA
SF3		 1Q57 Toth EA, Li Y, Sawaya MR, Cheng Y, Ellenberger T.:
The crystal structure of the bifunctional primase-helicase of bacteriophage T7
Mol. Cell 5 pp.1113-23 (2003)
         
UAP56
C-terminus		   RNA
SF2		 1T5I Zhao, R., Shen, J., Green, M. R., Macmorris, M., Blumenthal, T.:
Crystal Structure of Uap56, a "Dexd/H-Box" Protein Involved in Pre-Mrna Splicing and Mrna Export
Structure 12 pp. 1373 (2004)
         
UAP56
N-terminus		   RNA
SF2		 1T6N Zhao, R., Shen, J., Green, M. R., Macmorris, M., Blumenthal, T.:
Crystal Structure of Uap56, a "Dexd/H-Box" Protein Involved in Pre-Mrna Splicing and Mrna Export
Structure 12 pp. 1373 (2004)
         
UvrB   DNA
SF2		 1C4O Machius, M., Henry, L., Palnitkar, M., Deisenhofer, J.:
Crystal Structure of the DNA Nucleotide Excision Repair Enzyme Uvrb from Thermus Thermophilus
Proc.Nat.Acad.Sci.USA 96 pp. 11717 (1999)
         
UvrB   DNA
SF2		 1D2M   Nakagawa, N., Sugahara, M., Masui, R., Kato, R., Fukuyama, K.,Kuramitsu, S.:
Crystal Structure of Thermus Thermophilus Hb8 Uvrb Protein, a Key Enzyme of Nucleotide Excision Repair J. Biochem.(Tokyo) 126 pp. 986 (1999)
         
UvrB   DNA
SF2		 1D9X Theis, K., Chen, P. J., Skorvaga, M., Van Houten, B., Kisker, C.:
Crystal Structure of Uvrb, a DNA Helicase Adapted to Nucleotide Excision Repair
EMBO J. 18 pp. 6899 (1999)
         
UvrB ATP DNA
SF2		 1D9Z Theis, K., Chen, P. J., Skorvaga, M., Van Houten, B., Kisker, C.:
Crystal Structure of Uvrb, a DNA Helicase Adapted to Nucleotide Excision Repair
EMBO J. 18 pp. 6899 (1999)
         
UvsW     1RIF Sickmier, E. A., Kreuzer, K. N., White, S. W.:
The Crystal Structure of the Uvsw Helicase from Bacteriophage T4.
Structure 12 pp. 583 (2004)
         
         
list compiled by Maggie Fairman