Product Information “No Wash and Probenecid Cell Meter (TM) Free Endpoint Calcium Assay Kit * Optimized for Microplate Reading”
The probenecid Cell Meter (TM) no-wash end-point calcium assay kit enables homogeneous fluorescence-based assays to detect intracellular calcium mobilization mode without the need to use the kinetic readout. It can be used in lower reading mode fluorescence microplate readers that do not have a built-in liquid dispenser or kinetic reading capability.
After loading the Fluo-8E (TM) AM dye into the cells of interest, without washing steps, the calcium flux agonist can simply be added with a liquid dispenser or by hand pipetting, and then read the plate with a reader fluorescent at Ex / Em = 490/525 nm. Fluo-8E (TM) AM can passively cross the cell membrane by diffusion.
Once inside cells, the Fluo-8E (TM) AM lipophilic blocking groups are cleaved by esterase, resulting in a negatively charged fluorescent dye that remains within cells. Its fluorescence increases considerably and is long-lasting by binding to calcium. When cells expressing GPCRs of interest are stimulated with an agonist, the receptor signals intracellular calcium release, significantly increasing Fluo-8E (TM) fluorescence.
The characteristics of its high sensitivity,> 100-fold fluorescence magnification, and long-lasting fluorescent signal make Fluo-8E (TM) an ideal indicator for the measurement of cellular calcium in fluorescence microplate readers that do not have the ability to transfer. of fluids and kinetic reading mode. The Probenecid Cell Meter (TM) No Wash End-Point Calcium Assay Kit can be performed in a 96-well or 384-well microtiter plate format.
- Provider: AAT Bioquest
- Provider-Nr: 36312
- Format: dried
- Handling and safety
- Storage: -20 ° C (protect from light)
- Shipping: + 20 ° C (International: ° C)