High cell density culture of recombinant E. coli in the miniaturized bubble columns

High cell density culture of recombinant E. coli in the miniaturized bubble columns

Posted on by Laura
Miniaturized bubble columns (MBCs) can present mass switch traits much like stirred tank bioreactors. On this research, a brand new utility was developed for MBCs to analyze the impact of feeding technique and medium sort on the fed-batch tradition of recombinant E. coli. The outcomes confirmed that the exponential feeding technique and outlined M9 medium have been extra appropriate to attain the excessive cell density tradition (HCDC).
The utmost obtained cell focus in exponential feeding technique within the outlined medium with out induction, was at OD600 of 169, whereas glucose focus was maintained beneath 2 g/L. To one of the best of our information, this cell focus can’t be achieved in lab or pilot scale bubble columns. On the finish of the method, adversarial impact of the metabolic burden as a consequence of induction and mass switch limitations decreased the obtained closing cell focus to OD600 of 116.
Lastly, a comparability of the outcomes for fed-batch tradition within the stirred tank bioreactor with these of the MBCs confirmed that their decrease cell concentrations have been as a result of hydrodynamics limitations of MBCs. But, it was discovered that the MBCs are environment friendly instruments in growth of feeding methods and analysis of medium elements for HCDC of recombinant E. coli.

Expression and purification of purposeful recombinant CUL2•RBX1 from E. coli

Cullin-2 (CUL2) based mostly cullin-RING ligases (CRL2s) comprise a household of ubiquitin E3 ligases that exist solely in multi-cellular organisms and are essential for mobile processes akin to embryogenesis and viral pathogenesis. CUL2 is the scaffold protein that binds one of many interchangeable substrate receptor modules, which consists of adaptor proteins and the substrate receptor protein. The VHL protein is a substrate receptor identified to focus on hypoxia-inducible issue α (HIF1α) for ubiquitination and degradation.
Due to its crucial position within the ubiquitination of necessary mobile elements akin to HIF1α, CRL2s have been investigated for his or her organic capabilities and the event of novel therapeutics towards illnesses. Given the significance of CRL2s in organic and biomedical analysis, strategies that effectively produce purposeful CUL2 proteins will vastly facilitate research on the mechanism and regulation of CRL2s.
High cell density culture of recombinant E. coli in the miniaturized bubble columns
Right here, we report two cost-effective programs for the expression and purification of recombinant human CUL2 from E. coli cells. The purified CUL2 proteins have been ~ 95% pure, may bind their substrate receptor modules, and have been enzymatically energetic in transferring ubiquitin or ubiquitin-like protein to the corresponding substrate in in vitro assays. The offered methodological developments will assist advance analysis in CRL2 perform and regulation.

Milligram scale expression, refolding, and purification of Bombyx mori cocoonase utilizing a recombinant E. coli system

Silk is likely one of the most versatile biomaterials with signature properties of excellent mechanical energy and suppleness. A possible avenue for growing extra environmentally pleasant silk manufacturing is to utilize the silk moth (Bombyx mori) cocoonase, this may on the identical time improve the likelihood for utilizing the byproduct, sericin, as a uncooked materials for different purposes. Cocoonase is a serine protease utilized by the silk moth to melt the cocoon to allow its escape after accomplished metamorphosis.
Cocoonase selectively degrades the glue protein of the cocoon, sericin, with out affecting the silk-fiber product of the protein fibroin. Cocoonase will be recombinantly produced in E. coli, nevertheless, it’s solely discovered as insoluble inclusion our bodies. To resolve this drawback and to have the ability to make the most of the advantages related to an E. coli based mostly expression system, we have now developed a protocol that permits the manufacturing of soluble and purposeful protease within the milligram/liter scale. The core of the protocol is refolding of the protein in a buffer with a redox potential that’s optimized for formation of native and intramolecular di-sulfide bridges.
The redox potential was balanced with outlined concentrations of diminished and oxidized glutathione. This E coli based mostly manufacturing protocol will, along with construction dedication, additionally allow modification of cocoonase each by way of catalytic perform and stability. These elements will probably be precious elements within the growth of alternate silk manufacturing methodology.

Expression of Bacillus amyloliquefaciens transglutaminase in recombinant E. coli beneath the management of a bicistronic plasmid system in DO-stat fed-batch bioreactor cultivations

We studied the expression of Bacillus amyloliquefaciens transglutaminase cloned in Escherichia coli BL21(DE3)pLysS harboring the plasmid pBAD/3C/bTGase, a bicistronic expression system, in bioreactor cultivation. Batch and fed-batch managed as DO-stat methods have been employed for the manufacturing of the recombinant enzyme. In 30 h-batch cultivations utilizing Terrific broth (TB), 6 g/L of biomass and three.12 U/mgprotein of transglutaminase exercise have been obtained. DO-stat fed-batch cultivations beneath the management of oxygen focus (DO-stat) utilizing TB as medium however fed with glucose allowed the increment in biomass formation (17.5 g/L) and enzyme exercise (6.43 U/mgprotein).

TFF3 Human, Trefoil Factor-3 Human Recombinant Protein, His Tag
PROTQ07654-1 Regular: 20ug
EUR 317
Description: TFF3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids (22-80 a.a.) which includes a 10 amino acid His Tag fused at N-terminus and having a total molecular mass of 7.82 kDa. ;TFF3 Human Recombinant is purified by proprietary chromatographic techniques.

Recombinant Human Trefoil Factor-2
7-01588 5µg Ask for price

Recombinant Human Trefoil Factor-2
7-01589 20µg Ask for price

Recombinant Human Trefoil Factor-2
7-01590 1mg Ask for price

Recombinant Human Trefoil Factor-3
7-01594 5µg Ask for price

Recombinant Human Trefoil Factor-3
7-01595 20µg Ask for price

Recombinant Human Trefoil Factor-3
7-01596 1mg Ask for price

TFF1 Trefoil Factor-1 Human Recombinant Protein
PROTP04155 Regular: 20ug
EUR 317
Description: TFF-1 Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 60 amino acids which includes a 40 amino acid trefoil motif containing 3 conserved intramolecular disulfide bonds and having a total molecular mass of 13.2 kDa. ;TFF-1 Human Recombinant is purified by proprietary chromatographic techniques.

Recombinant Human Trefoil Factor-1 His Tag
7-01585 5µg Ask for price

Recombinant Human Trefoil Factor-1 His Tag
7-01586 20µg Ask for price

Recombinant Human Trefoil Factor-1 His Tag
7-01587 1mg Ask for price

TFF1, Trefoil Factor 1, human
RC712-25 5ug
EUR 104.38

Human Trefoil factor 1 (TFF1)
1-CSB-YP023431HU
  • EUR 430.00
  • EUR 234.00
  • EUR 1508.00
  • EUR 642.00
  • EUR 1009.00
  • EUR 291.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Trefoil factor 1(TFF1) expressed in Yeast

Human Trefoil factor 1 (TFF1)
1-CSB-EP023431HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Trefoil factor 1(TFF1) expressed in E.coli

Recombinant Human Trefoil Factor 1/TFF1 (C-6His)
C659-10ug 10ug
EUR 141
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human Trefoil Factor 1/TFF1 (C-6His)
C659-1mg 1mg
EUR 2283
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human Trefoil Factor 1/TFF1 (C-6His)
C659-500ug 500ug
EUR 1471
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human Trefoil Factor 1/TFF1 (C-6His)
C659-50ug 50ug
EUR 278
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Trefoil Factor 2 (TFF2)
4-RPA748Hu01
  • EUR 422.56
  • EUR 216.00
  • EUR 1309.60
  • EUR 503.20
  • EUR 906.40
  • EUR 346.00
  • EUR 3124.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Trefoil Factor 2 expressed in: E.coli

Recombinant Trefoil Factor 2 (TFF2)
4-RPA748Mu01
  • EUR 492.45
  • EUR 235.00
  • EUR 1571.68
  • EUR 590.56
  • EUR 1081.12
  • EUR 392.00
  • EUR 3779.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Trefoil Factor 2 expressed in: E.coli

Recombinant Trefoil Factor 2 (TFF2)
4-RPA748Ra01
  • EUR 512.16
  • EUR 240.00
  • EUR 1645.60
  • EUR 615.20
  • EUR 1130.40
  • EUR 406.00
  • EUR 3964.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Trefoil Factor 2 expressed in: E.coli

Human Trefoil Factor 1 (TFF1) Protein
20-abx069464
  • EUR 662.00
  • EUR 272.00
  • EUR 2040.00
  • EUR 787.00
  • EUR 481.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Trefoil Factor 1 (TFF1) Protein
20-abx260730
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Human Trefoil Factor 1 (TFF1) Protein
20-abx262086
  • EUR 4963.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Trefoil Factor 1 (TFF1) Antibody
20-abx130705
  • EUR 439.00
  • EUR 133.00
  • EUR 1247.00
  • EUR 592.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Trefoil Factor 1 (TFF1) Antibody
20-abx116256
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Trefoil Factor 1 (TFF1) Antibody
20-abx101588
  • EUR 411.00
  • EUR 133.00
  • EUR 1149.00
  • EUR 565.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Trefoil Factor 1 (TFF1) Antibody
20-abx101589
  • EUR 425.00
  • EUR 133.00
  • EUR 1177.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Trefoil Factor 1 (TFF1) Antibody
20-abx001483
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Trefoil Factor 1 (TFF1) Antibody
20-abx320288
  • EUR 300.00
  • EUR 244.00
  • 100 ul
  • 50 ul

Trefoil Factor 1 (TFF1) Antibody
abx218021-100ug 100 ug
EUR 425

Trefoil Factor 1 (TFF1) Antibody
20-abx339637
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Trefoil Factor 1 (TFF1) Antibody
abx238958-100ug 100 ug
EUR 551

Trefoil Factor 1 (TFF1) Antibody
20-abx213208
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Trefoil Factor 1 (TFF1) Antibody
20-abx174923
  • EUR 815.00
  • EUR 425.00
  • 1 mg
  • 200 ug

Trefoil Factor 1 (TFF1) Antibody
20-abx174924
  • EUR 871.00
  • EUR 453.00
  • 1 mg
  • 200 ug

Trefoil Factor 1 (TFF1) Antibody
abx330491-100ul 100 ul
EUR 425

anti- Trefoil factor 1 antibody
FNab08958 100µg
EUR 585
Description: Antibody raised against Trefoil factor 1

Anti-Trefoil factor 1 antibody
PAab08958 100 ug
EUR 412

Recombinant Human Trefoil Factor-2 His Tag
7-01591 5µg Ask for price

Recombinant Human Trefoil Factor-2 His Tag
7-01592 20µg Ask for price

Recombinant Human Trefoil Factor-2 His Tag
7-01593 1mg Ask for price

Recombinant Human Trefoil Factor-3 His Tag
7-01597 5µg Ask for price

Recombinant Human Trefoil Factor-3 His Tag
7-01598 20µg Ask for price

Recombinant Human Trefoil Factor-3 His Tag
7-01599 1mg Ask for price

TFF3 Trefoil Factor-3 Human Recombinant Protein
PROTQ07654 Regular: 20ug
EUR 317
Description: TFF-3 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 59 amino acid chains which includes a 40 amino acid trefoil motif containing 3 conserved interamolecular disulfide bonds and having a total molecular mass of 13.2kDa. ;TFF-3 Human Recombinant is purified by proprietary chromatographic techniques.

Recombinant Trefoil Factor 3, Intestinal (TFF3)
4-RPB656Bo01
  • EUR 528.29
  • EUR 244.00
  • EUR 1706.08
  • EUR 635.36
  • EUR 1170.72
  • EUR 416.00
  • EUR 4115.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Bovine Trefoil Factor 3, Intestinal expressed in: E.coli

Recombinant Trefoil Factor 3, Intestinal (TFF3)
4-RPB656Ca01
  • EUR 440.48
  • EUR 221.00
  • EUR 1376.80
  • EUR 525.60
  • EUR 951.20
  • EUR 358.00
  • EUR 3292.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Dog Trefoil Factor 3, Intestinal expressed in: E.coli

Recombinant Trefoil Factor 3, Intestinal (TFF3)
4-RPB656Hu01
  • EUR 481.70
  • EUR 232.00
  • EUR 1531.36
  • EUR 577.12
  • EUR 1054.24
  • EUR 385.00
  • EUR 3678.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Trefoil Factor 3, Intestinal expressed in: E.coli

Recombinant Trefoil Factor 3, Intestinal (TFF3)
4-RPB656Mu01
  • EUR 503.20
  • EUR 238.00
  • EUR 1612.00
  • EUR 604.00
  • EUR 1108.00
  • EUR 400.00
  • EUR 3880.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Trefoil Factor 3, Intestinal expressed in: E.coli

Recombinant Trefoil Factor 3, Intestinal (TFF3)
4-RPB656Mu02
  • EUR 503.20
  • EUR 238.00
  • EUR 1612.00
  • EUR 604.00
  • EUR 1108.00
  • EUR 400.00
  • EUR 3880.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Trefoil Factor 3, Intestinal expressed in: E.coli

Recombinant Trefoil Factor 3, Intestinal (TFF3)
4-RPB656Po01
  • EUR 535.46
  • EUR 246.00
  • EUR 1732.96
  • EUR 644.32
  • EUR 1188.64
  • EUR 421.00
  • EUR 4182.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Pig Trefoil Factor 3, Intestinal expressed in: E.coli

Recombinant Trefoil Factor 3, Intestinal (TFF3)
4-RPB656Po02
  • EUR 535.46
  • EUR 246.00
  • EUR 1732.96
  • EUR 644.32
  • EUR 1188.64
  • EUR 421.00
  • EUR 4182.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Pig Trefoil Factor 3, Intestinal expressed in: E.coli

Recombinant Trefoil Factor 3, Intestinal (TFF3)
4-RPB656Po03
  • EUR 535.46
  • EUR 246.00
  • EUR 1732.96
  • EUR 644.32
  • EUR 1188.64
  • EUR 421.00
  • EUR 4182.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Pig Recombinant Trefoil Factor 3, Intestinal (TFF3) expressed in: E.coli

Recombinant Trefoil Factor 3, Intestinal (TFF3)
4-RPB656Ra01
  • EUR 503.20
  • EUR 238.00
  • EUR 1612.00
  • EUR 604.00
  • EUR 1108.00
  • EUR 400.00
  • EUR 3880.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Trefoil Factor 3, Intestinal expressed in: E.coli

Human Trefoil Factor 1 (TFF1) ELISA Kit
20-abx153384
  • EUR 7112.00
  • EUR 3792.00
  • EUR 879.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Trefoil Factor 1 (TFF1) ELISA Kit
abx574326-96tests 96 tests
EUR 754

Human TFF1/ Trefoil factor 1 ELISA Kit
E2472Hu 1 Kit
EUR 571

Human Trefoil Factor 1 (TFF1) CLIA Kit
20-abx492361
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Trefoil Factor 1 (TFF1) ELISA Kit
abx252181-96tests 96 tests
EUR 754

Human Trefoil Factor 1 (TFF1) CLIA Kit
abx197843-96tests 96 tests
EUR 825

Human Trefoil Factor 1 (TFF1) ELISA Kit
DLR-TFF1-Hu-48T 48T
EUR 498
Description: A sandwich quantitative ELISA assay kit for detection of Human Trefoil Factor 1 (TFF1) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Trefoil Factor 1 (TFF1) ELISA Kit
DLR-TFF1-Hu-96T 96T
EUR 647
Description: A sandwich quantitative ELISA assay kit for detection of Human Trefoil Factor 1 (TFF1) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Trefoil Factor 1 ELISA Kit (TFF1)
RK02374 96 Tests
EUR 521

Human Trefoil Factor 1 (TFF1) ELISA Kit
RDR-TFF1-Hu-48Tests 48 Tests
EUR 522

Human Trefoil Factor 1 (TFF1) ELISA Kit
RDR-TFF1-Hu-96Tests 96 Tests
EUR 724

Human Trefoil Factor 1 (TFF1) ELISA Kit
RD-TFF1-Hu-48Tests 48 Tests
EUR 500

Human Trefoil Factor 1 (TFF1) ELISA Kit
RD-TFF1-Hu-96Tests 96 Tests
EUR 692

Human Trefoil Factor 1 (TFF1) Protein (Active)
20-abx655845
  • EUR 1066.00
  • EUR 398.00
  • EUR 3530.00
  • EUR 1288.00
  • EUR 732.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

ELISA kit for Human Trefoil factor 1
EK2642 96 tests
EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Trefoil factor 1 in samples from serum, plasma, tissue homogenates and other biological fluids.

Trefoil Factor 1 (TFF1) Polyclonal Antibody (Human)
4-PAB049Hu01
  • EUR 239.00
  • EUR 2391.00
  • EUR 598.00
  • EUR 299.00
  • EUR 211.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Trefoil Factor 1 (TFF1)

Trefoil Factor 1 (TFF1) Polyclonal Antibody (Human)
4-PAB049Hu02
  • EUR 239.00
  • EUR 2384.00
  • EUR 596.00
  • EUR 298.00
  • EUR 210.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Trefoil Factor 1 (TFF1)

Human Trefoil Factor 1 (TFF1) ELISA Kit
SEB049Hu-10x96wellstestplate 10x96-wells test plate
EUR 4502.43
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Trefoil Factor 1 (TFF1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Trefoil Factor 1 (TFF1) ELISA Kit
SEB049Hu-1x48wellstestplate 1x48-wells test plate
EUR 458.44
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Trefoil Factor 1 (TFF1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Trefoil Factor 1 (TFF1) ELISA Kit
SEB049Hu-1x96wellstestplate 1x96-wells test plate
EUR 612.05
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Trefoil Factor 1 (TFF1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Trefoil Factor 1 (TFF1) ELISA Kit
SEB049Hu-5x96wellstestplate 5x96-wells test plate
EUR 2454.23
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Trefoil Factor 1 (TFF1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Trefoil Factor 1 (TFF1) ELISA Kit
4-SEB049Hu
  • EUR 4553.00
  • EUR 2405.00
  • EUR 613.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Trefoil Factor 1 (TFF1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Trefoil Factor 1(TFF1)ELISA Kit
QY-E01704 96T
EUR 361

Human TFF1(Trefoil factor 1) ELISA Kit
EH0280 96T
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml

Human Trefoil factor 1, TFF1 ELISA KIT
ELI-03393h 96 Tests
EUR 824

TFF2, Trefoil Factor 2, human
RC712-26 5ug
EUR 104.38

Human Trefoil factor 3 (TFF3)
1-CSB-YP023433HU
  • EUR 504.00
  • EUR 265.00
  • EUR 1832.00
  • EUR 763.00
  • EUR 1216.00
  • EUR 334.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Trefoil factor 3(TFF3),partial expressed in Yeast

Mouse Trefoil Factor 1 (TFF1) Protein
20-abx069465
  • EUR 690.00
  • EUR 286.00
  • EUR 2124.00
  • EUR 815.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Trefoil Factor 1 (TFF1) Antibody Pair
20-abx370762
  • EUR 1706.00
  • EUR 1094.00
  • 10 × 96 tests
  • 5 × 96 tests

Trefoil Factor 1 (TFF1) Antibody Pair
20-abx370869
  • EUR 1414.00
  • EUR 913.00
  • 10 × 96 tests
  • 5 × 96 tests

Rat Trefoil Factor 1 (TFF1) Protein
20-abx168681
  • EUR 676.00
  • EUR 286.00
  • EUR 2082.00
  • EUR 801.00
  • EUR 481.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Trefoil Factor 1 (TFF1) Antibody (FITC)
20-abx271227
  • EUR 467.00
  • EUR 244.00
  • EUR 1358.00
  • EUR 648.00
  • EUR 356.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Trefoil Factor 1 (TFF1) Antibody (Biotin)
20-abx271493
  • EUR 439.00
  • EUR 244.00
  • EUR 1261.00
  • EUR 606.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Recombinant Human Trefoil Factor 2/TFF2 (C-6His)
C878-10ug 10ug
EUR 156
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.2.

Recombinant Human Trefoil Factor 2/TFF2 (C-6His)
C878-1mg 1mg
EUR 2283
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.2.

Recombinant Human Trefoil Factor 2/TFF2 (C-6His)
C878-500ug 500ug
EUR 1613
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.2.

Recombinant Human Trefoil Factor 2/TFF2 (C-6His)
C878-50ug 50ug
EUR 369
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.2.
DO-stat fed-batch utilizing mineral medium (M9) and fed with glucose beneath the identical circumstances produced even greater enzymatic exercise (9.14 U/mgprotein). The pH impact was investigated, and one of the best enzymatic exercise may very well be noticed at pH 8. In all cultivations, the bicistronic system remained secure, with 100% of plasmid-bearing cells. These outcomes present that E. coli bearing bicistronic plasmid constructs to precise recombinant TGase may very well be cultivated in bioreactors beneath DO-stat fed-batch utilizing mineral medium and it’s a promising technique in future optimizations to provide this necessary enzyme.

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