High cell density culture of recombinant E. coli in the miniaturized bubble columns

High cell density culture of recombinant E. coli in the miniaturized bubble columns

Posted on by Laura
Miniaturized bubble columns (MBCs) can present mass switch traits much like stirred tank bioreactors. On this research, a brand new utility was developed for MBCs to analyze the impact of feeding technique and medium sort on the fed-batch tradition of recombinant E. coli. The outcomes confirmed that the exponential feeding technique and outlined M9 medium have been extra appropriate to attain the excessive cell density tradition (HCDC).
The utmost obtained cell focus in exponential feeding technique within the outlined medium with out induction, was at OD600 of 169, whereas glucose focus was maintained beneath 2 g/L. To one of the best of our information, this cell focus can’t be achieved in lab or pilot scale bubble columns. On the finish of the method, adversarial impact of the metabolic burden as a consequence of induction and mass switch limitations decreased the obtained closing cell focus to OD600 of 116.
Lastly, a comparability of the outcomes for fed-batch tradition within the stirred tank bioreactor with these of the MBCs confirmed that their decrease cell concentrations have been as a result of hydrodynamics limitations of MBCs. But, it was discovered that the MBCs are environment friendly instruments in growth of feeding methods and analysis of medium elements for HCDC of recombinant E. coli.

Expression and purification of purposeful recombinant CUL2•RBX1 from E. coli

Cullin-2 (CUL2) based mostly cullin-RING ligases (CRL2s) comprise a household of ubiquitin E3 ligases that exist solely in multi-cellular organisms and are essential for mobile processes akin to embryogenesis and viral pathogenesis. CUL2 is the scaffold protein that binds one of many interchangeable substrate receptor modules, which consists of adaptor proteins and the substrate receptor protein. The VHL protein is a substrate receptor identified to focus on hypoxia-inducible issue α (HIF1α) for ubiquitination and degradation.
Due to its crucial position within the ubiquitination of necessary mobile elements akin to HIF1α, CRL2s have been investigated for his or her organic capabilities and the event of novel therapeutics towards illnesses. Given the significance of CRL2s in organic and biomedical analysis, strategies that effectively produce purposeful CUL2 proteins will vastly facilitate research on the mechanism and regulation of CRL2s.
High cell density culture of recombinant E. coli in the miniaturized bubble columns
Right here, we report two cost-effective programs for the expression and purification of recombinant human CUL2 from E. coli cells. The purified CUL2 proteins have been ~ 95% pure, may bind their substrate receptor modules, and have been enzymatically energetic in transferring ubiquitin or ubiquitin-like protein to the corresponding substrate in in vitro assays. The offered methodological developments will assist advance analysis in CRL2 perform and regulation.

Milligram scale expression, refolding, and purification of Bombyx mori cocoonase utilizing a recombinant E. coli system

Silk is likely one of the most versatile biomaterials with signature properties of excellent mechanical energy and suppleness. A possible avenue for growing extra environmentally pleasant silk manufacturing is to utilize the silk moth (Bombyx mori) cocoonase, this may on the identical time improve the likelihood for utilizing the byproduct, sericin, as a uncooked materials for different purposes. Cocoonase is a serine protease utilized by the silk moth to melt the cocoon to allow its escape after accomplished metamorphosis.
Cocoonase selectively degrades the glue protein of the cocoon, sericin, with out affecting the silk-fiber product of the protein fibroin. Cocoonase will be recombinantly produced in E. coli, nevertheless, it’s solely discovered as insoluble inclusion our bodies. To resolve this drawback and to have the ability to make the most of the advantages related to an E. coli based mostly expression system, we have now developed a protocol that permits the manufacturing of soluble and purposeful protease within the milligram/liter scale. The core of the protocol is refolding of the protein in a buffer with a redox potential that’s optimized for formation of native and intramolecular di-sulfide bridges.
The redox potential was balanced with outlined concentrations of diminished and oxidized glutathione. This E coli based mostly manufacturing protocol will, along with construction dedication, additionally allow modification of cocoonase each by way of catalytic perform and stability. These elements will probably be precious elements within the growth of alternate silk manufacturing methodology.

Expression of Bacillus amyloliquefaciens transglutaminase in recombinant E. coli beneath the management of a bicistronic plasmid system in DO-stat fed-batch bioreactor cultivations

We studied the expression of Bacillus amyloliquefaciens transglutaminase cloned in Escherichia coli BL21(DE3)pLysS harboring the plasmid pBAD/3C/bTGase, a bicistronic expression system, in bioreactor cultivation. Batch and fed-batch managed as DO-stat methods have been employed for the manufacturing of the recombinant enzyme. In 30 h-batch cultivations utilizing Terrific broth (TB), 6 g/L of biomass and three.12 U/mgprotein of transglutaminase exercise have been obtained. DO-stat fed-batch cultivations beneath the management of oxygen focus (DO-stat) utilizing TB as medium however fed with glucose allowed the increment in biomass formation (17.5 g/L) and enzyme exercise (6.43 U/mgprotein).

Recombinant Human Trefoil Factor-3
7-01596 1mg Ask for price

Recombinant Human Trefoil Factor-1 His Tag
7-01585 5µg Ask for price

Recombinant Human Trefoil Factor-1 His Tag
7-01586 20µg Ask for price

Recombinant Human Trefoil Factor-1 His Tag
7-01587 1mg Ask for price

Recombinant Trefoil Factor 1 (TFF1)
4-RPB049Hu01
  • EUR 571.58
  • EUR 276.00
  • EUR 1813.44
  • EUR 684.48
  • EUR 1248.96
  • EUR 458.40
  • EUR 4353.60
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Trefoil Factor 1 expressed in: E.coli

Recombinant Trefoil Factor 1 (TFF1)
4-RPB049Mu01
  • EUR 593.09
  • EUR 282.00
  • EUR 1894.08
  • EUR 711.36
  • EUR 1302.72
  • EUR 472.80
  • EUR 4555.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Trefoil Factor 1 expressed in: E.coli

Recombinant Trefoil Factor 1 (TFF1)
4-RPB049Ra01
  • EUR 582.34
  • EUR 279.60
  • EUR 1853.76
  • EUR 697.92
  • EUR 1275.84
  • EUR 465.60
  • EUR 4454.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Trefoil Factor 1 expressed in: E.coli

TFF1 Trefoil Factor-1 Human Recombinant Protein
PROTP04155 Regular: 20ug
EUR 380.4
Description: TFF-1 Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 60 amino acids which includes a 40 amino acid trefoil motif containing 3 conserved intramolecular disulfide bonds and having a total molecular mass of 13.2 kDa. ;TFF-1 Human Recombinant is purified by proprietary chromatographic techniques.

Recombinant Human Trefoil Factor 1/TFF1 (C-6His)
C659-10ug 10ug
EUR 169.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human Trefoil Factor 1/TFF1 (C-6His)
C659-1mg 1mg
EUR 2739.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human Trefoil Factor 1/TFF1 (C-6His)
C659-500ug 500ug
EUR 1765.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human Trefoil Factor 1/TFF1 (C-6His)
C659-50ug 50ug
EUR 333.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human Trefoil Factor-2 His Tag
7-01591 5µg Ask for price

Recombinant Human Trefoil Factor-2 His Tag
7-01592 20µg Ask for price

Recombinant Human Trefoil Factor-2 His Tag
7-01593 1mg Ask for price

Recombinant Human Trefoil Factor-3 His Tag
7-01597 5µg Ask for price

Recombinant Human Trefoil Factor-3 His Tag
7-01598 20µg Ask for price

Recombinant Human Trefoil Factor-3 His Tag
7-01599 1mg Ask for price

Trefoil Factor 1 Recombinant Protein
40-292-0005mg 0.005 mg
EUR 311.1
Description: The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are expressed in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF1 is essential for normal differentiation of the antral and pyloric gastric mucosa and functions as a gastric-specific tumor suppressor gene. Recombinant human TFF1 is a 6.7 kDa monomeric protein consisting of a 60 amino acid polypeptide chain, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.

Trefoil Factor 1 Recombinant Protein
40-292-002mg 0.02 mg
EUR 437.1
Description: The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are expressed in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF1 is essential for normal differentiation of the antral and pyloric gastric mucosa and functions as a gastric-specific tumor suppressor gene. Recombinant human TFF1 is a 6.7 kDa monomeric protein consisting of a 60 amino acid polypeptide chain, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.

Recombinant Human Trefoil Factor 2/TFF2 (C-6His)
C878-10ug 10ug
EUR 187.2
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.2.

Recombinant Human Trefoil Factor 2/TFF2 (C-6His)
C878-1mg 1mg
EUR 2739.6
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.2.

Recombinant Human Trefoil Factor 2/TFF2 (C-6His)
C878-500ug 500ug
EUR 1935.6
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.2.

Recombinant Human Trefoil Factor 2/TFF2 (C-6His)
C878-50ug 50ug
EUR 442.8
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.2.

TFF2 Trefoil Factor-2 Human Recombinant Protein
PROTQ03403-1 Regular: 20ug
EUR 380.4
Description: TFF-2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 106 amino acids (24-129) and having a total molecular mass of 12 kDa. TFF2 Human Recombinant includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds and was purified by proprietary chromatographic techniques.

TFF3 Trefoil Factor-3 Human Recombinant Protein
PROTQ07654 Regular: 20ug
EUR 380.4
Description: TFF-3 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 59 amino acid chains which includes a 40 amino acid trefoil motif containing 3 conserved interamolecular disulfide bonds and having a total molecular mass of 13.2kDa. ;TFF-3 Human Recombinant is purified by proprietary chromatographic techniques.

Recombinant Trefoil Factor 2 (TFF2)
4-RPA748Hu01
  • EUR 507.07
  • EUR 259.20
  • EUR 1571.52
  • EUR 603.84
  • EUR 1087.68
  • EUR 415.20
  • EUR 3748.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Trefoil Factor 2 expressed in: E.coli

Recombinant Trefoil Factor 2 (TFF2)
4-RPA748Mu01
  • EUR 590.94
  • EUR 282.00
  • EUR 1886.02
  • EUR 708.67
  • EUR 1297.34
  • EUR 470.40
  • EUR 4535.04
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Trefoil Factor 2 expressed in: E.coli

Recombinant Trefoil Factor 2 (TFF2)
4-RPA748Ra01
  • EUR 614.59
  • EUR 288.00
  • EUR 1974.72
  • EUR 738.24
  • EUR 1356.48
  • EUR 487.20
  • EUR 4756.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Trefoil Factor 2 expressed in: E.coli

TFF1 Human, Trefoil Factor-1 Human Recombinant Protein, His Tag
PROTP04155-1 Regular: 20ug
EUR 380.4
Description: TFF-1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids (25-84) which includes a 10 amino acid His Tag and having a total molecular mass of 7.9 kDa. ;TFF-1 Human Recombinant is purified by proprietary chromatographic techniques.

Trefoil Factor 2 Recombinant Protein
40-291-0005mg 0.005 mg
EUR 311.1
Description: The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are expressed in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF2 has been shown to inhibit gastrointestinal motility and gastric acid secretion. Recent data suggests a potential role for TFF2 in acute and chronic asthma (Nikolaidis, N.M. et al. Am. Journal Respir. Cell Mol. Biol. (2003) 4: 458-464). Recombinant human TFF2 is a 12.0 kDa polypeptide of 106 amino acid residues, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.

Trefoil Factor 2 Recombinant Protein
40-291-002mg 0.02 mg
EUR 437.1
Description: The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are expressed in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF2 has been shown to inhibit gastrointestinal motility and gastric acid secretion. Recent data suggests a potential role for TFF2 in acute and chronic asthma (Nikolaidis, N.M. et al. Am. Journal Respir. Cell Mol. Biol. (2003) 4: 458-464). Recombinant human TFF2 is a 12.0 kDa polypeptide of 106 amino acid residues, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.

Trefoil Factor 3 Recombinant Protein
40-293-0005mg 0.005 mg
EUR 311.1
Description: The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are secreted in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF-3 is expressed by goblet cells and in the uterus, and has also been shown to express in certain cancers, including colorectal, hepatocellular, and in biliary tumors. TFF3 may be useful as a molecular marker for certain types of cancer, but its role, if any, in tumorigenesis is unknown. TFF3 also promotes airway epithelial cell migration and differentiation. Recombinant human TFF3 is a 13.2 kDa homodimeric protein consisting of two 59 amino acid chains, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.

Trefoil Factor 3 Recombinant Protein
40-293-002mg 0.02 mg
EUR 437.1
Description: The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are secreted in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF-3 is expressed by goblet cells and in the uterus, and has also been shown to express in certain cancers, including colorectal, hepatocellular, and in biliary tumors. TFF3 may be useful as a molecular marker for certain types of cancer, but its role, if any, in tumorigenesis is unknown. TFF3 also promotes airway epithelial cell migration and differentiation. Recombinant human TFF3 is a 13.2 kDa homodimeric protein consisting of two 59 amino acid chains, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.

Trefoil Factor 2 Recombinant Protein
91-669 0.05 mg
EUR 651.3
Description: Trefoil Factor 2 (TFF2) is a member of the trefoil family and contains two P-type (trefoil) domains. Members of this family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. TFF2 is a secreted protein and specifically expressed in the stomach. TFF2 inhibits gastrointestinal motility and gastric acid secretion. TFF2 could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains.

TFF2 Human, Trefoil Factor-2 Human Recombinant Protein, His Tag
PROTQ03403 Regular: 20ug
EUR 380.4
Description: TFF-2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 116 amino acids (24-129) which includes a 10 amino acid His Tag fused at N-terminus and having a total molecular mass of 13.2 kDa. ;TFF2 Human Recombinant is purified by proprietary chromatographic techniques.

TFF3 Human, Trefoil Factor-3 Human Recombinant Protein, His Tag
PROTQ07654-1 Regular: 20ug
EUR 380.4
Description: TFF3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids (22-80 a.a.) which includes a 10 amino acid His Tag fused at N-terminus and having a total molecular mass of 7.82 kDa. ;TFF3 Human Recombinant is purified by proprietary chromatographic techniques.

Recombinant Human Trefoil factor 2 Protein, Untagged, E.coli-5ug
QP10179-ec-5ug 5ug
EUR 184.8

Recombinant Trefoil Factor 3, Intestinal (TFF3)
4-RPB656Bo01
  • EUR 633.95
  • EUR 292.80
  • EUR 2047.30
  • EUR 762.43
  • EUR 1404.86
  • EUR 499.20
  • EUR 4938.24
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Bovine Trefoil Factor 3, Intestinal expressed in: E.coli

Recombinant Trefoil Factor 3, Intestinal (TFF3)
4-RPB656Ca01
  • EUR 528.58
  • EUR 265.20
  • EUR 1652.16
  • EUR 630.72
  • EUR 1141.44
  • EUR 429.60
  • EUR 3950.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Dog Trefoil Factor 3, Intestinal expressed in: E.coli

Recombinant Trefoil Factor 3, Intestinal (TFF3)
4-RPB656Hu01
  • EUR 578.04
  • EUR 278.40
  • EUR 1837.63
  • EUR 692.54
  • EUR 1265.09
  • EUR 462.00
  • EUR 4414.08
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Trefoil Factor 3, Intestinal expressed in: E.coli

Recombinant Trefoil Factor 3, Intestinal (TFF3)
4-RPB656Mu01
  • EUR 603.84
  • EUR 285.60
  • EUR 1934.40
  • EUR 724.80
  • EUR 1329.60
  • EUR 480.00
  • EUR 4656.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Trefoil Factor 3, Intestinal expressed in: E.coli

Recombinant Trefoil Factor 3, Intestinal (TFF3)
4-RPB656Mu02
  • EUR 603.84
  • EUR 285.60
  • EUR 1934.40
  • EUR 724.80
  • EUR 1329.60
  • EUR 480.00
  • EUR 4656.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Trefoil Factor 3, Intestinal expressed in: E.coli

Recombinant Trefoil Factor 3, Intestinal (TFF3)
4-RPB656Po01
  • EUR 642.55
  • EUR 295.20
  • EUR 2079.55
  • EUR 773.18
  • EUR 1426.37
  • EUR 505.20
  • EUR 5018.88
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Pig Trefoil Factor 3, Intestinal expressed in: E.coli

Recombinant Trefoil Factor 3, Intestinal (TFF3)
4-RPB656Po02
  • EUR 642.55
  • EUR 295.20
  • EUR 2079.55
  • EUR 773.18
  • EUR 1426.37
  • EUR 505.20
  • EUR 5018.88
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Pig Trefoil Factor 3, Intestinal expressed in: E.coli

Recombinant Trefoil Factor 3, Intestinal (TFF3)
4-RPB656Po03
  • EUR 642.55
  • EUR 295.20
  • EUR 2079.55
  • EUR 773.18
  • EUR 1426.37
  • EUR 505.20
  • EUR 5018.88
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Pig Recombinant Trefoil Factor 3, Intestinal (TFF3) expressed in: E.coli

Recombinant Trefoil Factor 3, Intestinal (TFF3)
4-RPB656Ra01
  • EUR 603.84
  • EUR 285.60
  • EUR 1934.40
  • EUR 724.80
  • EUR 1329.60
  • EUR 480.00
  • EUR 4656.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Trefoil Factor 3, Intestinal expressed in: E.coli

Recombinant Human Trefoil factor 2 Protein, Untagged, E.coli-20ug
QP10179-ec-20ug 20ug
EUR 348

Recombinant Human Trefoil factor 2 Protein, Untagged, E.coli-100ug
QP10179-ec-100ug 100ug
EUR 1185.6

Recombinant Human Trefoil factor 2 Protein, Untagged, E.coli-250ug
QP10179-ec-250ug 250ug
EUR 2077.2

TFF3 Trefoil Factor-3 Rat Recombinant Protein
PROTQ03191 Regular: 10ug
EUR 380.4
Description: The Trefoil Factor-3 Rat was constructed as a recombinant protein with a 9 a.a C-terminal fusion of Flag-Tag (1 aa N-terminal+8 aa C-terminal). The TFF3 Rat produced in E.coli, is 7.7kDa protein containing a total of 68 amino acid residues.

Recombinant (E. coli) Human Trefoil factor 1 (TFF1) protein control for WB
TFF12-C 100 ul
EUR 343.2

Recombinant Human pS2/ pNR-2/ Trefoil Factor 1 Protein, Untagged, E.coli-20ug
QP10178-ec-20ug 20ug
EUR 348

Recombinant Human pS2/ pNR-2/ Trefoil Factor 1 Protein, Untagged, E.coli-5ug
QP10178-ec-5ug 5ug
EUR 184.8

Recombinant Human TFF3/ Trefoil Factor 3 Protein, Untagged, E.coli-5ug
QP10180-ec-5ug 5ug
EUR 184.8

Recombinant Mouse Trefoil Factor 2/TFF2 (C-6His)
CU56-10ug 10ug
EUR 157.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse Trefoil Factor 2/TFF2 (C-6His)
CU56-1mg 1mg
EUR 2008.8
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse Trefoil Factor 2/TFF2 (C-6His)
CU56-500ug 500ug
EUR 1399.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse Trefoil Factor 2/TFF2 (C-6His)
CU56-50ug 50ug
EUR 327.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse Trefoil Factor 3/TFF3 (C-6His)
C661-10ug 10ug
EUR 169.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse Trefoil Factor 3/TFF3 (C-6His)
C661-1mg 1mg
EUR 2739.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse Trefoil Factor 3/TFF3 (C-6His)
C661-500ug 500ug
EUR 1765.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse Trefoil Factor 3/TFF3 (C-6His)
C661-50ug 50ug
EUR 333.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human pS2/ pNR-2/ Trefoil Factor 1 Protein, Untagged, E.coli-100ug
QP10178-ec-100ug 100ug
EUR 1185.6

Recombinant Human pS2/ pNR-2/ Trefoil Factor 1 Protein, Untagged, E.coli-250ug
QP10178-ec-250ug 250ug
EUR 2077.2

Recombinant Human TFF3/ Trefoil Factor 3 Protein, Untagged, E.coli-100ug
QP10180-ec-100ug 100ug
EUR 1185.6

Recombinant Human TFF3/ Trefoil Factor 3 Protein, Untagged, E.coli-20ug
QP10180-ec-20ug 20ug
EUR 348

Recombinant Human TFF3/ Trefoil Factor 3 Protein, Untagged, E.coli-250ug
QP10180-ec-250ug 250ug
EUR 2077.2

Recombinant (E. coli) Human Trefoil factor 2 (TFF2) protein control for WB
TFF22-C 100 ul
EUR 343.2

Human Trefoil factor 1 (TFF1)
1-CSB-YP023431HU
  • EUR 516.00
  • EUR 280.80
  • EUR 1809.60
  • EUR 770.40
  • EUR 1210.80
  • EUR 349.20
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Trefoil factor 1(TFF1) expressed in Yeast

Human Trefoil factor 1 (TFF1)
1-CSB-EP023431HU
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Trefoil factor 1(TFF1) expressed in E.coli

TFF1, Trefoil Factor 1, human
RC712-25 5ug
EUR 125.26

Human Trefoil Factor 1 ELISA kit
E01T0132-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Trefoil Factor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Trefoil Factor 1 ELISA kit
E01T0132-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Trefoil Factor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Trefoil Factor 1 ELISA kit
E01T0132-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Trefoil Factor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Trefoil Factor 1 (TFF1) Protein
20-abx260730
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Human Trefoil Factor 1 (TFF1) Protein
20-abx262086
  • EUR 5955.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Human Trefoil Factor 1 (TFF1) Protein
20-abx069464
  • EUR 794.40
  • EUR 326.40
  • EUR 2448.00
  • EUR 944.40
  • EUR 577.20
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Trefoil factor 3 (TFF3)
1-CSB-YP023433HU
  • EUR 604.80
  • EUR 318.00
  • EUR 2198.40
  • EUR 915.60
  • EUR 1459.20
  • EUR 400.80
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Trefoil factor 3(TFF3),partial expressed in Yeast

Human Trefoil Factor 1 (TFF1) CLIA Kit
abx197843-96tests 96 tests
EUR 990

Human Trefoil Factor 1 (TFF1) CLIA Kit
20-abx492361
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

ELISA kit for Human Trefoil factor 1
EK2642 96 tests
EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Trefoil factor 1 in samples from serum, plasma, tissue homogenates and other biological fluids.

TFF2, Trefoil Factor 2, human
RC712-26 5ug
EUR 125.26

Human Trefoil Factor ELISA kit
E01T0871-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Human Trefoil Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Trefoil Factor ELISA kit
E01T0871-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Human Trefoil Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Trefoil Factor ELISA kit
E01T0871-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Human Trefoil Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Trefoil Factor 1 (TFF1) ELISA Kit
abx252181-96tests 96 tests
EUR 904.8

Human Trefoil Factor 1 (TFF1) ELISA Kit
abx574326-96tests 96 tests
EUR 904.8

Human Trefoil Factor 1 (TFF1) ELISA Kit
DLR-TFF1-Hu-48T 48T
EUR 597.6
Description: A sandwich quantitative ELISA assay kit for detection of Human Trefoil Factor 1 (TFF1) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Trefoil Factor 1 (TFF1) ELISA Kit
DLR-TFF1-Hu-96T 96T
EUR 776.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Trefoil Factor 1 (TFF1) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Trefoil Factor 1 (TFF1) ELISA Kit
20-abx153384
  • EUR 8534.40
  • EUR 4550.40
  • EUR 1054.80
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Trefoil factor 1, TFF1 ELISA KIT
ELI-03393h 96 Tests
EUR 988.8

Human Trefoil Factor 1 ELISA Kit (TFF1)
RK02374 96 Tests
EUR 625.2

Human Trefoil Factor 1 (TFF1) ELISA Kit
RDR-TFF1-Hu-48Tests 48 Tests
EUR 626.4

Human Trefoil Factor 1 (TFF1) ELISA Kit
RDR-TFF1-Hu-96Tests 96 Tests
EUR 868.8

Human Trefoil Factor 1 (TFF1) ELISA Kit
RD-TFF1-Hu-48Tests 48 Tests
EUR 600

Human Trefoil Factor 1 (TFF1) ELISA Kit
RD-TFF1-Hu-96Tests 96 Tests
EUR 830.4

Human Trefoil Factor 1(TFF1)ELISA Kit
QY-E01704 96T
EUR 433.2

Human Trefoil Factor 1 (TFF1) ELISA Kit
SEB049Hu-10x96wellstestplate 10x96-wells test plate
EUR 5402.92
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Trefoil Factor 1 (TFF1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Trefoil Factor 1 (TFF1) ELISA Kit
SEB049Hu-1x48wellstestplate 1x48-wells test plate
EUR 550.13
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Trefoil Factor 1 (TFF1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Trefoil Factor 1 (TFF1) ELISA Kit
SEB049Hu-1x96wellstestplate 1x96-wells test plate
EUR 734.46
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Trefoil Factor 1 (TFF1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Trefoil Factor 1 (TFF1) ELISA Kit
SEB049Hu-5x96wellstestplate 5x96-wells test plate
EUR 2945.08
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Trefoil Factor 1 (TFF1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Trefoil Factor 1 (TFF1) ELISA Kit
4-SEB049Hu
  • EUR 5463.60
  • EUR 2886.00
  • EUR 735.60
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Trefoil Factor 1 (TFF1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Trefoil Factor 1 (TFF1) ELISA Kit
RDR-TFF1-Hu-48T 48T
EUR 453.92
Description: serum, plasma, tissue homogenates and other biological fluids.

Human Trefoil Factor 1 (TFF1) ELISA Kit
RDR-TFF1-Hu-96T 96T
EUR 648.38
Description: serum, plasma, tissue homogenates and other biological fluids.

Human Trefoil Factor 1 (TFF1) ELISA Kit
RD-TFF1-Hu-48T 48T
EUR 432.3
Description: serum, plasma, tissue homogenates and other biological fluids.

Human Trefoil Factor 1 (TFF1) ELISA Kit
RD-TFF1-Hu-96T 96T
EUR 617.5
Description: serum, plasma, tissue homogenates and other biological fluids.

Human TFF1/ Trefoil factor 1 ELISA Kit
E2472Hu 1 Kit
EUR 685.2

Human TFF1(Trefoil factor 1) ELISA Kit
EH0280 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml

Human Trefoil Factor 1 (TFF1) Protein (Active)
20-abx655845
  • EUR 1279.20
  • EUR 477.60
  • EUR 4236.00
  • EUR 1545.60
  • EUR 878.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Trefoil Factor 2 ELISA kit
E01I0120-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Trefoil Factor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Trefoil Factor 2 ELISA kit
E01I0120-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Trefoil Factor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Trefoil Factor 2 ELISA kit
E01I0120-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Trefoil Factor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

CLIA kit for Human TFF1 (Trefoil Factor 1)
E-CL-H1159 1 plate of 96 wells
EUR 700.8
Description: A sandwich CLIA kit for quantitative measurement of Human TFF1 (Trefoil Factor 1) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human TFF1 (Trefoil Factor 1)
E-EL-H1872 1 plate of 96 wells
EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Human TFF1 (Trefoil Factor 1) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human TFF1 (Trefoil Factor 1)
ELK1746 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Trefoil Factor 1 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Human Trefoil Factor 2 (TFF2) Protein
20-abx260736
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Human Trefoil Factor 3 (TFF3) Protein
20-abx261607
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Human Trefoil Factor 2 (TFF2) Protein
20-abx262087
  • EUR 5955.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Human Trefoil Factor 3 (TFF3) Protein
20-abx262088
  • EUR 5955.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Human Trefoil Factor 2 (TFF2) Protein
20-abx069467
  • EUR 710.40
  • EUR 309.60
  • EUR 2131.20
  • EUR 844.80
  • EUR 526.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Trefoil Factor 1 (TFF1) Polyclonal Antibody (Human)
4-PAB049Hu01
  • EUR 286.80
  • EUR 2869.20
  • EUR 717.60
  • EUR 358.80
  • EUR 253.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Trefoil Factor 1 (TFF1)

Trefoil Factor 1 (TFF1) Polyclonal Antibody (Human)
4-PAB049Hu02
  • EUR 286.80
  • EUR 2860.80
  • EUR 715.20
  • EUR 357.60
  • EUR 252.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Trefoil Factor 1 (TFF1)

Trefoil Factor 1 (TFF1) Antibody
abx218021-100ug 100 ug
EUR 510

Trefoil Factor 1 (TFF1) Antibody
abx238958-100ug 100 ug
EUR 661.2

Trefoil Factor 1 (TFF1) Antibody
20-abx174923
  • EUR 978.00
  • EUR 510.00
  • 1 mg
  • 200 ug

Trefoil Factor 1 (TFF1) Antibody
20-abx174924
  • EUR 1045.20
  • EUR 543.60
  • 1 mg
  • 200 ug

Trefoil Factor 1 (TFF1) Antibody
20-abx213208
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Trefoil Factor 1 (TFF1) Antibody
20-abx116256
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Trefoil Factor 1 (TFF1) Antibody
20-abx130705
  • EUR 526.80
  • EUR 159.60
  • EUR 1496.40
  • EUR 710.40
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Trefoil Factor 1 (TFF1) Antibody
abx330491-100ul 100 ul
EUR 510

Trefoil Factor 1 (TFF1) Antibody
20-abx101588
  • EUR 493.20
  • EUR 159.60
  • EUR 1378.80
  • EUR 678.00
  • EUR 376.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Trefoil Factor 1 (TFF1) Antibody
20-abx101589
  • EUR 510.00
  • EUR 159.60
  • EUR 1412.40
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Trefoil Factor 1 (TFF1) Antibody
20-abx320288
  • EUR 360.00
  • EUR 292.80
  • 100 ul
  • 50 ul

Trefoil Factor 1 (TFF1) Antibody
20-abx339637
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Trefoil Factor 1 (TFF1) Antibody
20-abx001483
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

anti- Trefoil factor 1 antibody
FNab08958 100µg
EUR 702
Description: Antibody raised against Trefoil factor 1

Human Trefoil Factor 2 (TFF2) CLIA Kit
abx196275-96tests 96 tests
EUR 990

Human Trefoil Factor 2 (TFF2) CLIA Kit
20-abx491979
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

ELISA kit for Human Trefoil factor 2
EK2202 96 tests
EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Trefoil factor 2 in samples from serum, plasma, tissue homogenates and other biological fluids.
DO-stat fed-batch utilizing mineral medium (M9) and fed with glucose beneath the identical circumstances produced even greater enzymatic exercise (9.14 U/mgprotein). The pH impact was investigated, and one of the best enzymatic exercise may very well be noticed at pH 8. In all cultivations, the bicistronic system remained secure, with 100% of plasmid-bearing cells. These outcomes present that E. coli bearing bicistronic plasmid constructs to precise recombinant TGase may very well be cultivated in bioreactors beneath DO-stat fed-batch utilizing mineral medium and it’s a promising technique in future optimizations to provide this necessary enzyme.

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