Skip to content
DExH/D protein family db Helicase Enzymes

DExH/D protein family db Helicase Enzymes

E. coli recombinant human Protein for normal cell culture

Menu
  • Blog
  • Controls
  • Cryo
  • Control
  • Specificity
  • Conventional
  • Cow
  • Contact Us
  • Distributors
Menu
Selective Release of Recombinant Periplasmic Protein From E. coli Using Continuous Pulsed Electric Field Treatment

Selective Release of Recombinant Periplasmic Protein From E. coli Using Continuous Pulsed Electric Field Treatment

Posted on July 9, 2021July 1, 2021 by Laura
To this point, high-pressure homogenization is the usual technique for cell disintegration earlier than the extraction of cytosolic and periplasmic protein from E. coli. Its most important disadvantage, nevertheless, is low selectivity and a ensuing excessive load of host cell impurities. Pulsed electrical subject (PEF) therapy could also be used for selective permeabilization of the outer membrane. PEF is a course of which is ready to generate pores inside cell membranes, the so-called electroporation. It may be readily utilized to the tradition broth in steady mode, no extra chemical compounds are wanted, warmth technology is comparatively low, and it’s already applied at industrial scale within the meals sector.
But, research about PEF-assisted extraction of recombinant protein from micro organism are scarce. Within the current examine, steady electroporation was employed to selectively extract recombinant Protein A from the periplasm of E. coli. For this function, a particularly designed flow-through PEF therapy chamber was deployed, operated at 1.5 kg/h, utilizing rectangular pulses of three μs at particular vitality enter ranges between 10.Three and 241.9 kJ/kg. Power enter was managed by variation of the electrical subject power (28.4-44.eight kV/cm) and pulse repetition frequency (50-1,000 Hz). The consequences of the method parameters on cell viability, product launch, and host cell protein (HCP), DNA, in addition to endotoxin (ET) masses have been investigated.
It was discovered {that a} most product launch of 89% was achieved with growing vitality enter ranges. Cell loss of life additionally progressively elevated, with a most inactivation of -0.9 log at 241.9 kJ/kg. The circumstances leading to excessive launch efficiencies whereas maintaining impurities low have been electrical subject strengths ≤ 30 kV/cm and frequencies ≥ 825 Hz. Compared with high-pressure homogenization, PEF therapy resulted in 40% much less HCP load, 96% much less DNA load, and 43% much less ET load. Subsequently, PEF therapy may be an environment friendly different to the cell disintegration processes generally utilized in downstream processing.

Technology of cell-permeant recombinant human transcription issue GATA4 from E. coli

Transcription issue GATA4 is expressed throughout early embryogenesis and is significant for correct growth. As well as, it’s a essential reprogramming issue for deriving purposeful cardiomyocytes and was just lately recognized as a tumor suppressor protein in numerous cancers. To generate a secure and efficient molecular instrument that may probably be utilized in a cell reprogramming course of and as an anti-cancer agent, we have now recognized optimum expression parameters to acquire soluble expression of human GATA4 in E. coli and purified the identical to homogeneity below native circumstances utilizing immobilized metallic ion affinity chromatography. The identification of GATA4 protein was confirmed utilizing western blotting and mass spectrometry. Utilizing round dichroism spectroscopy, it was demonstrated that the purified recombinant protein has maintained its secondary construction, primarily comprising of random coils and α-helices. Subsequently, this purified recombinant protein was utilized to human cells and was discovered that it was non-toxic and in a position to enter the cells in addition to translocate to the nucleus. Prospectively, this cell- and nuclear-permeant molecular instrument is appropriate for cell reprogramming experiments and generally is a secure and efficient therapeutic agent for most cancers remedy.

Immobilization of Recombinant E. coli Cells in a Bacterial Cellulose-Silk Composite Matrix To Protect Organic Operate

Methods for the encapsulation of cells for the design of cell-based sensors require environment friendly immobilization procedures whereas preserving organic exercise of the reporter cells. Right here, we introduce an immobilization method that depends upon the symbiotic relationship between two bacterial strains: cellulose-producing Gluconacetobacter xylinus cells; and recombinant Escherichia coli cells harboring recombinase-based dual-color artificial riboswitch (RS), as a mannequin for cell-based sensor. Following sequential coculturing of recombinant cells within the cellulose matrix, last immobilization of E. coli cells was accomplished after reconstituted silk fibroin (SF) protein was added to a “dwelling membrane” producing the composite bacterial cellulose-silk fibroin (BC-SF) scaffold.
 Selective Release of Recombinant Periplasmic Protein From E. coli Using Continuous Pulsed Electric Field Treatment
By controlling incubation parameters for each sorts of cells, in addition to the conformations in SF secondary construction, a wide range of sturdy composite scaffolds have been ready starting from opaque to clear. The properties of the scaffolds have been in contrast by way of porosity, water capability, distribution of recombinant cells throughout the scaffolds matrix, onset of cells activation, and skill to guard recombinant operate of cells in opposition to UV irradiation.
The closer-fitted microstructure of clear BC-SF scaffolds resulted in leakage-free encapsulation of recombinant cells with preserved RS operate due to a mixture of a number of parameters that carefully matched properties of a biofilm setting. Together with correct elasticity, wonderful porosity, capability to retain the water, and skill of SF to soak up UV mild, the composite hydrogel materials offered needed circumstances to kind confined cell colonies that changed cell metabolism and enhanced cell resilience to the stresses induced by encapsulation.

Purification of recombinant human chemokine CCL2 in E. coli and its operate in ovarian most cancers

Chemokine (CC-motif) ligand 2 (CCL2) is an inflammatory cytokine that regulates the infiltration and migration of monocytes. It’s extremely expressed by each tumor and stromal cells and has been related to tumorigenesis. Nonetheless, the impact of the exogenous administration of CCL2 on ovarian most cancers stays largely unknown. On this report, we tried to determine an expression system in Escherichia coli to supply recombinant hCCL2. The recombinant plasmid containing the hCCL2 cDNA was ready utilizing the prokaryotic-expression plasmid pGEX-5X-Three and reworked into E. coli BL21. GST-hCCL2 was efficiently induced by 0.1 mmol/L IPTG at 20 °C for six h, and the recombinant protein was purified utilizing affinity chromatography.

LCK, Active Human Recombinant Protein

GWB-CBBFF1 GenWay Biotech 10ug Ask for price

ITK, Active Human Recombinant Protein

GWB-BE69DC GenWay Biotech 10ug Ask for price

BTK, Active Human Recombinant Protein

GWB-6B7FE7 GenWay Biotech 10ug Ask for price

Ret, Active Human Recombinant Protein

GWB-1E9110 GenWay Biotech 10ug Ask for price

Bas, Active Human Recombinant Protein

GWB-PS58BD GenWay Biotech 20ug Ask for price

FES, Active Human Recombinant Protein

GWB-PS9783 GenWay Biotech 10ug Ask for price

FMS, Active Human Recombinant Protein

GWB-149EA7 GenWay Biotech 10ug Ask for price

CSK, Active Human Recombinant Protein

GWB-175A88 GenWay Biotech 10ug Ask for price

CHK2, Active Human Recombinant Protein

GWB-8B3EF8 GenWay Biotech 10ug Ask for price

XIAP, Active Human Recombinant Protein

GWB-E21113 GenWay Biotech 20ug Ask for price

Rheb, Active Human Recombinant Protein

GWB-A769CA GenWay Biotech 100ug Ask for price

Her4, Active Human Recombinant Protein

GWB-B2354A GenWay Biotech 10ug Ask for price

TRKB, Active Human Recombinant Protein

GWB-B34C75 GenWay Biotech 10ug Ask for price

DPP4, Active Human Recombinant Protein

GWB-C138A0 GenWay Biotech 10ug Ask for price

cRAF, Active Human Recombinant Protein

GWB-C565DD GenWay Biotech 10ug Ask for price

MTAP, Active Human Recombinant Protein

GWB-C6140F GenWay Biotech 50ug Ask for price

Flt1, Active Human Recombinant Protein

GWB-C71D7F GenWay Biotech 10ug Ask for price

DPP3, Active Human Recombinant Protein

GWB-BB089E GenWay Biotech 10ug Ask for price

Akt1,Active Human Recombinant Protein

GWB-BB3CA2 GenWay Biotech 10ug Ask for price

Her2, Active Human Recombinant Protein

GWB-BDFB0E GenWay Biotech 10ug Ask for price

Jak3, Active Human Recombinant Protein

GWB-BE5317 GenWay Biotech 10ug Ask for price

PLK3, Active Human Recombinant Protein

GWB-BFCE77 GenWay Biotech 10ug Ask for price

ACK1, Active Human Recombinant Protein

GWB-D2EC8D GenWay Biotech 10ug Ask for price

ROS1 Active Human Recombinant Protein

GWB-D47510 GenWay Biotech 10ug Ask for price

NEK6, Active Human Recombinant Protein

GWB-D5655E GenWay Biotech 10ug Ask for price

AHCY, Active Human Recombinant Protein

GWB-CEAD99 GenWay Biotech 50ug Ask for price

p85a, Active Human Recombinant Protein

GWB-48E60C GenWay Biotech 100ug Ask for price

PLK1, Active Human Recombinant Protein

GWB-55E483 GenWay Biotech 10ug Ask for price

NEK2, Active Human Recombinant Protein

GWB-571315 GenWay Biotech 10ug Ask for price

aRAF, Active Human Recombinant Protein

GWB-5D1440 GenWay Biotech 10ug Ask for price

DPP8, Active Human Recombinant Protein

GWB-5D9292 GenWay Biotech 10ug Ask for price

MKK6 Active Human Recombinant Protein

GWB-6247F2 GenWay Biotech 20ug Ask for price

WWP1, Active Human Recombinant Protein

GWB-64C08F GenWay Biotech 20ug Ask for price

DPP9, Active Human Recombinant Protein

GWB-662992 GenWay Biotech 10ug Ask for price

DPP7, Active Human Recombinant Protein

GWB-66D491 GenWay Biotech 10ug Ask for price

Akt3, Active Human Recombinant Protein

GWB-676CAF GenWay Biotech 10ug Ask for price

Flt3, Active Human Recombinant Protein

GWB-2DC31A GenWay Biotech 10ug Ask for price

TRKC, Active Human Recombinant Protein

GWB-2E1DAB GenWay Biotech 10ug Ask for price

Aha1, Active Human Recombinant Protein

GWB-331CAB GenWay Biotech 200ug Ask for price

Tie2, Active Human Recombinant Protein

GWB-341504 GenWay Biotech 10ug Ask for price

Tyk2 Active Human Recombinant Protein

GWB-35FD7B GenWay Biotech 10ug Ask for price

DDR2 Active Human Recombinant Protein

GWB-370F00 GenWay Biotech 0.01 mg Ask for price

MUSK, Active Human Recombinant Protein

GWB-6FDFEA GenWay Biotech 10ug Ask for price

EGFR, Active Human Recombinant Protein

GWB-767301 GenWay Biotech 10ug Ask for price

PDK1, Active Human Recombinant Protein

GWB-7E6CB9 GenWay Biotech 10ug Ask for price

MDM2 Active Human Recombinant Protein

GWB-1EA32E GenWay Biotech 20ug Ask for price

TRKA, Active Human Recombinant Protein

GWB-43AF13 GenWay Biotech 10ug Ask for price

Jak1 Active Human Recombinant Protein

GWB-PS3ABB GenWay Biotech 10ug Ask for price

NEK7, Active Human Recombinant Protein

GWB-PS7203 GenWay Biotech 10ug Ask for price

USP7, Active Human Recombinant Protein

GWB-PS99CD GenWay Biotech 100ug Ask for price

NEK9, Active Human Recombinant Protein

GWB-PSA505 GenWay Biotech 10ug Ask for price

TDP2, Active Human Recombinant Protein

GWB-PSAAD3 GenWay Biotech 10ug Ask for price

PrCP, Active Human Recombinant Protein

GWB-PSC500 GenWay Biotech 10ug Ask for price

cMet, Active Human Recombinant Protein

GWB-PSD799 GenWay Biotech 10ug Ask for price

PKM1, Active Human Recombinant Protein

GWB-PSE52C GenWay Biotech 20ug Ask for price

cKit, Active Human Recombinant Protein

GWB-PSF7CE GenWay Biotech 10ug Ask for price

PYK2, Active Human Recombinant Protein

GWB-87F170 GenWay Biotech 10ug Ask for price

PLK2, Active Human Recombinant Protein

GWB-16CD9F GenWay Biotech 10ug Ask for price

PDE1C, Active Human Recombinant Protein

GWB-886086 GenWay Biotech 10ug Ask for price

FGFR2, Active Human Recombinant Protein

GWB-D9F2A2 GenWay Biotech 10ug Ask for price

EphA2, Active Human Recombinant Protein

GWB-DC8558 GenWay Biotech 10ug Ask for price

PDE1A, Active Human Recombinant Protein

GWB-E0AF30 GenWay Biotech 10ug Ask for price

PDE3A, Active Human Recombinant Protein

GWB-AF80BD GenWay Biotech 10ug Ask for price

IRAK4, Active Human Recombinant Protein

GWB-B993EE GenWay Biotech 10ug Ask for price

USP14 Active Human Recombinant Protein

GWB-FADC72 GenWay Biotech 50ug Ask for price

DAPK1 Active Human Recombinant Protein

GWB-C2E2A4 GenWay Biotech 10ug Ask for price

PDE7B, Active Human Recombinant Protein

GWB-C35AC2 GenWay Biotech 10ug Ask for price

USP10 Active Human Recombinant Protein

GWB-D66F05 GenWay Biotech 50ug Ask for price

PDE3B, Active Human Recombinant Protein

GWB-495C2E GenWay Biotech 10ug Ask for price

PDE1B, Active Human Recombinant Protein

GWB-50CAF7 GenWay Biotech 10ug Ask for price

DYRK2 Active Human Recombinant Protein

GWB-63A6A5 GenWay Biotech 10ug Ask for price

EphB2, Active Human Recombinant Protein

GWB-6A2C5D GenWay Biotech 10ug Ask for price

STK33 Active Human Recombinant Protein

GWB-6A33CB GenWay Biotech 10ug Ask for price

EphB4, Active Human Recombinant Protein

GWB-6ABD60 GenWay Biotech 10ug Ask for price

ZAP70, Active Human Recombinant Protein

GWB-3C3FB6 GenWay Biotech 10ug Ask for price

Hsp40, Active Human Recombinant Protein

GWB-71D83F GenWay Biotech 100ug Ask for price

NEDD4, Active Human Recombinant Protein

GWB-79E152 GenWay Biotech 20ug Ask for price

PDE7A, Active Human Recombinant Protein

GWB-2500BE GenWay Biotech 10ug Ask for price

BRSK2, Active Human Recombinant Protein

GWB-267372 GenWay Biotech 10ug Ask for price

PDE6C, Active Human Recombinant Protein

GWB-1CE561 GenWay Biotech 10ug Ask for price

VPS34 Active Human Recombinant Protein

GWB-7FA3C0 GenWay Biotech 20ug Ask for price

UCHL3 Active Human Recombinant Protein

GWB-819E99 GenWay Biotech 100ug Ask for price

PTPmu, Active Human Recombinant Protein

GWB-PS0EEC GenWay Biotech 20ug Ask for price

MEKK2, Active Human Recombinant Protein

GWB-PS4378 GenWay Biotech 10ug Ask for price

FGFR4, Active Human Recombinant Protein

GWB-PS501A GenWay Biotech 10ug Ask for price

RPTPg, Active Human Recombinant Protein

GWB-PS67C2 GenWay Biotech 20ug Ask for price

RNF20 Active Human Recombinant Protein

GWB-PSA6BC GenWay Biotech 20ug Ask for price

UBE3A Active Human Recombinant Protein

GWB-PSF951 GenWay Biotech 20ug Ask for price

ALAS2, Active Human Recombinant Protein

GWB-10D327 GenWay Biotech 100ug Ask for price

PDGFRb, Active Human Recombinant Protein

GWB-8C1FA1 GenWay Biotech 10ug Ask for price

PDE5A1, Active Human Recombinant Protein

GWB-9C8952 GenWay Biotech 10ug Ask for price

CoREST, Active Human Recombinant Protein

GWB-EBBBD1 GenWay Biotech 100ug Ask for price

SMURF1, Active Human Recombinant Protein

GWB-FF1056 GenWay Biotech 20ug Ask for price

Hsp90b, Active Human Recombinant Protein

GWB-C657EE GenWay Biotech 200ug Ask for price

PDE4C1, Active Human Recombinant Protein

GWB-C9515C GenWay Biotech 5ug Ask for price

FKBP12, Active Human Recombinant Protein

GWB-D2E2E9 GenWay Biotech 200ug Ask for price

PDE4D3, Active Human Recombinant Protein

GWB-48E7AE GenWay Biotech 5ug Ask for price

VEGFR3, Active Human Recombinant Protein

GWB-52D140 GenWay Biotech 10ug Ask for price

p70S6K, Active Human Recombinant Protein

GWB-531A58 GenWay Biotech 20ug Ask for price

SMURF2, Active Human Recombinant Protein

GWB-5581F8 GenWay Biotech 20ug Ask for price

PDE8A1, Active Human Recombinant Protein

GWB-60E572 GenWay Biotech 10ug Ask for price

PDE4D2, Active Human Recombinant Protein

GWB-63994F GenWay Biotech 0.005 mg Ask for price

ALOX15 Active Human Recombinant Protein

GWB-6672E3 GenWay Biotech 20ug Ask for price

PDE4D7, Active Human Recombinant Protein

GWB-6754B8 GenWay Biotech 5ug Ask for price

RbAp48, Active Human Recombinant Protein

GWB-74261D GenWay Biotech 50ug Ask for price

PDE4B2, Active Human Recombinant Protein

GWB-3ED046 GenWay Biotech 5ug Ask for price

PDGFRa Active Human Recombinant Protein

GWB-24DADD GenWay Biotech 0.01 mg Ask for price

Hsp90a, Active Human Recombinant Protein

GWB-816F04 GenWay Biotech 200ug Ask for price

OTUD6B Active Human Recombinant Protein

GWB-PS2F91 GenWay Biotech 50ug Ask for price

PDE4D2, Active Human Recombinant Protein

GWB-PS819C GenWay Biotech 5ug Ask for price

PDE4B1, Active Human Recombinant Protein

GWB-87851A GenWay Biotech 10ug Ask for price

PDE9A2, Active Human Recombinant Protein

GWB-15988D GenWay Biotech 10ug Ask for price

PDE10A2 Active Human Recombinant Protein

GWB-A6F078 GenWay Biotech 10ug Ask for price

PDE4A1A, Active Human Recombinant Protein

GWB-4DB22F GenWay Biotech 10ug Ask for price

PDE10A1 Active Human Recombinant Protein

GWB-2AAF78 GenWay Biotech 10ug Ask for price

PDE4A10 Active Human Recombinant Protein

GWB-PS90FE GenWay Biotech 10ug Ask for price

PDE4A4B Active Human Recombinant Protein

GWB-PSF5A3 GenWay Biotech 10ug Ask for price

PTPbeta, Active Human Recombinant Protein

GWB-PSFF36 GenWay Biotech 20ug Ask for price

PDE11A4, Active Human Recombinant Protein

GWB-11830F GenWay Biotech 10ug Ask for price

PKG1beta, Active Human Recombinant Protein

GWB-64BEAA GenWay Biotech 10ug Ask for price

GSK3beta, Active Human Recombinant Protein

GWB-683604 GenWay Biotech 10ug Ask for price

GSK3alpha, Active Human Recombinant Protein

GWB-FCAF97 GenWay Biotech 10ug Ask for price

PKG1alpha, Active Human Recombinant Protein

GWB-23A92A GenWay Biotech 10ug Ask for price

FYN A, Active Human Recombinant Protein

GWB-PS6991 GenWay Biotech 10ug Ask for price

Bcl-2, Active Human Recombinant Protein

GWB-532360 GenWay Biotech 100ug Ask for price

ACC-2, Active Human Recombinant Protein

GWB-79EAC4 GenWay Biotech 10ug Ask for price

ACC-1, Active Human Recombinant Protein

GWB-2009DC GenWay Biotech 10ug Ask for price

MEG-2, Active Human Recombinant Protein

GWB-PS0DE2 GenWay Biotech 20ug Ask for price

ACC-1, Active Human Recombinant Protein

GWB-PS2090 GenWay Biotech 10ug Ask for price

MEG-1, Active Human Recombinant Protein

GWB-PSB90D GenWay Biotech 20ug Ask for price

Galactokinase, Active Human Recombinant Protein

GWB-4FB715 GenWay Biotech 200ug Ask for price

BRAF/p50, Active Human Recombinant Protein

GWB-932C16 GenWay Biotech 10ug Ask for price

HDAC-9, Active Human Recombinant Protein

GWB-EAC877 GenWay Biotech 10ug Ask for price

Cot/Tpl2, Active Human Recombinant Protein

GWB-AF0BA2 GenWay Biotech 10ug Ask for price

IGF-1R, Active Human Recombinant Protein

GWB-AF1F98 GenWay Biotech 10ug Ask for price

Bcl-xL, Active Human Recombinant Protein

GWB-B2AE2B GenWay Biotech 100ug Ask for price

Jak2 Jh1, Active Human Recombinant Protein

GWB-FDAB1C GenWay Biotech 10ug Ask for price

HDAC-8, Active Human Recombinant Protein

GWB-C3AA2A GenWay Biotech 50ug Ask for price

HDAC-5, Active Human Recombinant Protein

GWB-C87EF0 GenWay Biotech 10ug Ask for price

HDAC-7, Active Human Recombinant Protein

GWB-675906 GenWay Biotech 10ug Ask for price

USP2 (Sf9) Active Human Recombinant Protein

GWB-1D6F15 GenWay Biotech 50ug Ask for price

HDAC-4, Active Human Recombinant Protein

GWB-3CD9B3 GenWay Biotech 10ug Ask for price

HDAC-1, Active Human Recombinant Protein

GWB-222284 GenWay Biotech 50ug Ask for price

MNK1 (5385D), Active Human Recombinant Protein

GWB-18F7F0 GenWay Biotech 10ug Ask for price

PDE2A (GST) Active Human Recombinant Protein

GWB-A3CBB9 GenWay Biotech 5ug Ask for price

UBE1 (UBA1), Active Human Recombinant Protein

GWB-ACB42F GenWay Biotech 100ug Ask for price

HDAC-11, Active Human Recombinant Protein

GWB-B569A2 GenWay Biotech 50ug Ask for price

USP5 (IsoT) Active Human Recombinant Protein

GWB-F64EB7 GenWay Biotech 50ug Ask for price

USP8 (UBPY) Active Human Recombinant Protein

GWB-FD384C GenWay Biotech 50ug Ask for price

STK3(MST2) Active Human Recombinant Protein

GWB-CFDEDF GenWay Biotech 10ug Ask for price

LAR (PTPRF), Active Human Recombinant Protein

GWB-PS0392 GenWay Biotech 20ug Ask for price

UbcH7 (Sf9) Active Human Recombinant Protein

GWB-PS7B04 GenWay Biotech 50ug Ask for price

STK4(MST1) Active Human Recombinant Protein

GWB-140C39 GenWay Biotech 10ug Ask for price

Lok1/Stk10, Active Human Recombinant Protein

GWB-88ED64 GenWay Biotech 10ug Ask for price

UCHL1 (PGP9.5) Active Human Recombinant Protein

GWB-8B6CAA GenWay Biotech 100ug Ask for price

Sirtuin 3, Active Human Recombinant Protein

GWB-9B6EDF GenWay Biotech 100ug Ask for price

GCN5 (KAT2A) Active Human Recombinant Protein

GWB-A0A7F0 GenWay Biotech 0.1 mg Ask for price

Sirtuin 2, Active Human Recombinant Protein

GWB-A5C283 GenWay Biotech 100ug Ask for price

LSD1 (KDM1A), Active Human Recombinant Protein

GWB-DA8CBF GenWay Biotech 50ug Ask for price

VEGFR2/KDR, Active Human Recombinant Protein

GWB-DFAF3A GenWay Biotech 10ug Ask for price

NcoR2/SMRT, Active Human Recombinant Protein

GWB-A9C2CB GenWay Biotech 50ug Ask for price

Abl Kinase Active Human Recombinant Protein

GWB-AC67E0 GenWay Biotech 10ug Ask for price

EGFR (T790M), Active Human Recombinant Protein

GWB-B8F694 GenWay Biotech 10ug Ask for price

EGFR (L858R), Active Human Recombinant Protein

GWB-C7EA4F GenWay Biotech 10ug Ask for price

Sirtuin 7, Active Human Recombinant Protein

GWB-CCF2E4 GenWay Biotech 100ug Ask for price

cKit (V654A), Active Human Recombinant Protein

GWB-D22242 GenWay Biotech 10ug Ask for price

Sirtuin 4, Active Human Recombinant Protein

GWB-D38420 GenWay Biotech 100ug Ask for price

PDE2A (FLAG), Active Human Recombinant Protein

GWB-568389 GenWay Biotech 5ug Ask for price

cKit (V560G), Active Human Recombinant Protein

GWB-3C026F GenWay Biotech 10ug Ask for price

Sirtuin 6, Active Human Recombinant Protein

GWB-77B41E GenWay Biotech 100ug Ask for price

Sirtuin 5, Active Human Recombinant Protein

GWB-7CBD91 GenWay Biotech 100ug Ask for price

cKit (D816V), Active Human Recombinant Protein

GWB-261D48 GenWay Biotech 10ug Ask for price

FGFR1(FLT2) Active Human Recombinant Protein

GWB-2B8F61 GenWay Biotech 0.01 mg Ask for price

CD45(PTPRC), Active Human Recombinant Protein

GWB-PS017F GenWay Biotech 20ug Ask for price

p300 (KAT3B) Active Human Recombinant Protein

GWB-PSF0ED GenWay Biotech 50ug Ask for price

DEP1(PTPRJ), Active Human Recombinant Protein

GWB-PSF58A GenWay Biotech 20ug Ask for price

Active Recombinant Human RIPK1 protein, GST-tagged, Active

RIPK1-202H Creative BioMart 5ug
EUR 558.4
Description: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Jak2 Jh1Jh2, Active Human Recombinant Protein

GWB-96F2A5 GenWay Biotech 10ug Ask for price

PDE4C (mouse) Active Human Recombinant Protein

GWB-9E15AE GenWay Biotech 0.005 mg Ask for price

UBA6 (UBE1L2), Active Human Recombinant Protein

GWB-CA6D99 GenWay Biotech 100ug Ask for price

MAP3K14(NIK), Active Human Recombinant Protein

GWB-309564 GenWay Biotech 10ug Ask for price

HDAC10 (FLAG) Active Human Recombinant Protein

GWB-21102D GenWay Biotech 50ug Ask for price

MAPK10 (JNK3), Active Human Recombinant Protein

GWB-80410B GenWay Biotech 10ug Ask for price

PEST (PTPN12), Active Human Recombinant Protein

GWB-PS6D56 GenWay Biotech 20ug Ask for price

Cathepsin V, Active Human Recombinant Protein

GWB-C384A7 GenWay Biotech 10ug Ask for price

Cathepsin B, Active Human Recombinant Protein

GWB-BACD47 GenWay Biotech 10ug Ask for price

Cathepsin L, Active Human Recombinant Protein

GWB-CECF6C GenWay Biotech 10ug Ask for price

UbcH13 (UBE2N) Active Human Recombinant Protein

GWB-4979C0 GenWay Biotech 100ug Ask for price

UEV1A (UBE2V1) Active Human Recombinant Protein

GWB-52C044 GenWay Biotech 100ug Ask for price

PTPIA2(PTPRN), Active Human Recombinant Protein

GWB-5T9WX1 GenWay Biotech 20ug Ask for price

Cathepsin F, Active Human Recombinant Protein

GWB-653508 GenWay Biotech 50ug Ask for price

Cathepsin S, Active Human Recombinant Protein

GWB-6B460A GenWay Biotech 10ug Ask for price

UbcH6 (UBE2E1) Active Human Recombinant Protein

GWB-2B4BDF GenWay Biotech 100ug Ask for price

UbcH5a (UBE2D1) Active Human Recombinant Protein

GWB-22F0FC GenWay Biotech 100ug Ask for price

CDK6/cyclinD3, Active Human Recombinant Protein

GWB-802AFF GenWay Biotech 20ug Ask for price

SYK, Catalytic, Active Human Recombinant Protein

GWB-PS3961 GenWay Biotech 10ug Ask for price

CDK3/CyclinE1, Active Human Recombinant Protein

GWB-PSD2F9 GenWay Biotech 10ug Ask for price

CDK2/CyclinA2, Active Human Recombinant Protein

GWB-PSF6FD GenWay Biotech 10ug Ask for price

PTPsigma/PTPRS, Active Human Recombinant Protein

GWB-PSEF32 GenWay Biotech 20ug Ask for price
×
The purified protein was recognized by SDS-PAGE and Western Blot. In vitro experiments revealed that rhCCL2 promoted the proliferation of ovarian most cancers cells and elevated the degrees of phosphorylation of MEK and ERK1/2, and the degrees of JUN, RELB and NF-κB2 mRNA. Moreover, inhibition of ERK signaling by therapy with PD98059 decreased ovarian most cancers cell proliferation and ranges of JUN, RELB, and NF-κB2 mRNA, indicating that exogenous rhCCL2 elevated the proliferation of ovarian most cancers cells, partially by activating the MAPK/ERK pathway, and by focusing on JUN, RELB, and NF-κB2. Our examine uncovered a selling position of exogenous CCL2 on ovarian most cancers cell proliferation by way of the MAPK/ERK signaling pathway, which can facilitate the invention of extra potential roles of CCL2 in ovarian most cancers.

Leave a Reply Cancel reply

Your email address will not be published. Required fields are marked *

Recent Posts

  • From Laboratory to Clinic: The Role of Glycoproteomics in Next-Generation Drug Development
  • Exploiting the capability of Glycan-Protein Associations: An advanced method in Vaccine Design
  • Navigating Carbohydrate-Protein Interaction Platforms: Revolutionizing Therapeutic Innovation and Innovation
  • Investigating Glycan Structures with Mass Spectrometric Analysis: A revolutionary development in Bioconjugation and Pharmaceutical Development
  • Glycosylation and Its Influence on mAbs: Improving Therapeutic Potential Through Glycoengineering

Categories

  • Blog
  • Control
  • Controls
  • Conventional
  • Cow
  • Cryo
  • Specificity

Tags

agrobacterium fabrum agrobacterium mediated gene transfer agrobacterium mediated gene transfer ppt agrobacterium spp agrobacterium ti plasmid agrobacterium transformation protocol agrobacterium tumefaciens pdf agrobacterium tumefaciens symptoms agrobacterium tumefaciens transformation agrobacterium tumefaciens treatment corticosterone elisa kit cotinine elisa kit dna dna replication elisa assay kit elisa kits manufacturer elisa test kits estradiol elisa kit human humana humana.com humana.com login humana login humana military humana military login humana pharmacy humanapharmacy.com humana pharmacy login human benchmark humane society animal shelter humane society near me humane society tacoma human genome project human interface devices humanism humanity humanity login humankind humanoid human resources human rights watch human services human tnf alpha elisa kit il-6 elisa kit lassa fever igg elisa kit

Categories

  • Blog
  • Control
  • Controls
  • Conventional
  • Cow
  • Cryo
  • Specificity

Recent Posts

  • From Laboratory to Clinic: The Role of Glycoproteomics in Next-Generation Drug Development
  • Exploiting the capability of Glycan-Protein Associations: An advanced method in Vaccine Design
  • Navigating Carbohydrate-Protein Interaction Platforms: Revolutionizing Therapeutic Innovation and Innovation
  • Investigating Glycan Structures with Mass Spectrometric Analysis: A revolutionary development in Bioconjugation and Pharmaceutical Development
  • Glycosylation and Its Influence on mAbs: Improving Therapeutic Potential Through Glycoengineering

Tags

agrobacterium fabrum agrobacterium mediated gene transfer agrobacterium mediated gene transfer ppt agrobacterium spp agrobacterium ti plasmid agrobacterium transformation protocol agrobacterium tumefaciens pdf agrobacterium tumefaciens symptoms agrobacterium tumefaciens transformation agrobacterium tumefaciens treatment corticosterone elisa kit cotinine elisa kit dna dna replication elisa assay kit elisa kits manufacturer elisa test kits estradiol elisa kit human humana humana.com humana.com login humana login humana military humana military login humana pharmacy humanapharmacy.com humana pharmacy login human benchmark humane society animal shelter humane society near me humane society tacoma human genome project human interface devices humanism humanity humanity login humankind humanoid human resources human rights watch human services human tnf alpha elisa kit il-6 elisa kit lassa fever igg elisa kit
© 2025 DExH/D protein family db Helicase Enzymes | Powered by Superbs Personal Blog theme