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Selective Release of Recombinant Periplasmic Protein From E. coli Using Continuous Pulsed Electric Field Treatment
To this point, high-pressure homogenization is the usual technique for cell disintegration earlier than the extraction of cytosolic and periplasmic protein from E. coli. Its most important disadvantage, nevertheless, is low selectivity and a ensuing excessive load of host cell impurities. Pulsed electrical subject (PEF) therapy could also be used for selective permeabilization of the outer… Continue reading