Fucoidan oligosaccharides possesses numerous physicochemical and organic actions. Particular glycoside hydrolases are beneficial instruments for degrading polysaccharides to supply oligosaccharides. On this examine, BcFucA, a novel fucosidase belonging to GH95 household from Bacillus cereus 2-8, was cloned into pET21a vector, expressed in E. coli BL21 (DE3) and characterised. The protein consists of 1136 amino acid residues encoded by 3411 bases and has a molecular weight of 125.35 kDa. The optimum temperature and pH of this enzyme are 50°C and pH 4.0. As well as, this examine confirmed that the unknown perform area (encoding Lys261-Thr681) outlined as a linker is sort of necessary for its exercise. The obtained novel enzyme BcFucA will contribute to the efficient degradation of fucoidan and future industrial purposes.
Optimization of medium composition to extend the expression of <em>recombinant</em> human interferon-β utilizing the Plackett-Burman and central composite design in <i><em>E</em>. <em>coli</em></i> S<em>E</em>1
Recombinant human interferon-β (rhIFN-β) is therapeutically necessary and new commercially viable approaches are wanted for its elevated manufacturing. On this examine, a codon-optimized gene encoding for rhIFN-β(C17S) protein was designed and expressed in E. coli SE1. As a primary step of medium optimization, progress of E. coli as a perform of various media parts was studied. Subsequently, to optimize the media composition, a response floor methodology (RSM) was used.
Our outcomes present that optimized medium (15.Zero g/L tryptone, 12.three g/L meat extract, 1.Zero g/L MgSO4 and 0.5 g/L thiamine together with minimal medium) obtained on this examine present higher progress of recombinant cells and the expression degree of recombinant protein was ~ 1.7-fold greater than Luria-Bertani medium. The optimized medium could also be utilized for the large-scale manufacturing of rhIFN-β.
Purposeful analysis of a recombinant fungal immunomodulatory protein from L. rhinocerus produced in P. pastoris and E. coli host expression techniques
Fungal immunomodulatory proteins (FIPs) are bioactive proteins with immunomodulatory properties. We beforehand reported the heterologous manufacturing in Escherichia coli of FIP-Lrh from Tiger milk mushroom (Lignosus rhinocerus) with potent cytotoxic impact on most cancers cell strains. Nonetheless, protein produced in E. coli lacks post-translational modifications and could also be contaminated with lipopolysaccharide (LPS) endotoxin. Due to this fact, on this examine, yFIP-Lrh produced in Pichia pastoris was functionally in contrast with eFIP-Lrh produced in E. coli.
Expression assemble of FIP-Lrh cDNA in pPICZα was generated, reworked into P. pastoris X-33 and Mut+ transformants have been verified by colony PCR. Induction with 0.5% or 1% methanol resulted in a secreted 13.6 kDa yFIP-Lrh which was subsequently purified and verified utilizing LCMS/MS evaluation. Measurement exclusion chromatography confirmed eFIP-Lrh as a homodimer whereas the bigger measurement of yFIP-Lrh could point out post-translational modification regardless of damaging for glycoproteins staining.
At decrease focus (4-Eight μg/mL), yFIP-Lrh induced considerably larger Th1 (IFN-γ, TNF-α) and Th2 (IL-6, IL-4, IL-5, IL-13) cytokines manufacturing in mice splenocytes, whereas 16 μg/mL eFIP-Lrh induced considerably larger pro-inflammatory cytokines (TNF-α, IL-6, IL-10), probably attributable to larger residual LPS endotoxin (0.082 EU/mL) in eFIP-Lrh in comparison with negligible degree in yFIP-Lrh (0.001 EU/mL).
Moreover, yFIP-Lrh confirmed larger cytotoxic impact on MCF-7 and HeLa most cancers cells. Since each recombinant proteins of FIP-Lrh have the identical peptide sequence, moreover glycosylation, different post-translational modifications in yFIP-Lrh could account for its enhanced immunomodulatory and anti-proliferative actions.
In conclusion, P. pastoris is most popular over E. coli for manufacturing of a functionally energetic yFIP-Lrh devoid of endotoxin contamination. KEY POINTS: • FIP-Lrh can induced manufacturing of Th1 and Th2 cytokines by mouse splenocytes. • Larger cytotoxic impact on most cancers cells noticed for yeast in comparison with E. coli produced FIP-Lrh. • P. pastoris permits manufacturing of an endotoxin-free and functionally energetic recombinant FIP-Lrh.
Endolysin-Primarily based Autolytic E. coli System for Facile Restoration of Recombinant Proteins
Restoration of recombinant proteins from the Escherichia coli cytoplasm will depend on cell disruption by mechanical, chemical, and/or enzymatic strategies, which normally trigger incomplete cell breakage or protein denaturation. Controllable autolytic E. coli strains have been designed to facilitate the purification of recombinant proteins; nevertheless, these strains undergo from low restoration yield, sluggish cell lysis, or in depth pressure engineering. Herein, we report an improved, extremely environment friendly programmable autolytic E. coli platform, through which cell lysis is initiated upon the induced expression of T4 lysozyme with N-terminal fusion of a cell-penetrating peptide.
Recombinant Thrombopoietin (TPO) |
RPA135Ca01 |
Cloud-Clone |
10ug |
EUR 196 |
Recombinant Thrombopoietin (TPO) |
RPA135Ga01 |
Cloud-Clone |
10ug |
EUR 176 |
Recombinant Thrombopoietin (TPO) |
RPA135Hu01 |
Cloud-Clone |
10ug |
EUR 164 |
Recombinant Thrombopoietin (TPO) |
RPA135Hu02 |
Cloud-Clone |
10ug |
EUR 160 |
Recombinant Thrombopoietin (TPO) |
RPA135Hu03 |
Cloud-Clone |
10ug |
EUR 166 |
Recombinant Thrombopoietin (TPO) |
RPA135Hu04 |
Cloud-Clone |
10ug |
EUR 165 |
Recombinant Thrombopoietin (TPO) |
RPA135Mu01 |
Cloud-Clone |
10ug |
EUR 140 |
Recombinant Thrombopoietin (TPO) |
RPA135Po01 |
Cloud-Clone |
10ug |
EUR 164 |
Recombinant Thrombopoietin (TPO) |
RPA135Po02 |
Cloud-Clone |
10ug |
EUR 166 |
Recombinant Thrombopoietin (TPO) |
RPA135Po03 |
Cloud-Clone |
10ug |
EUR 166 |
Recombinant Thrombopoietin (TPO) |
RPA135Ra01 |
Cloud-Clone |
10ug |
EUR 176 |
TPO Thrombopoietin Human Recombinant Protein |
PROTP40225-1 |
BosterBio |
Regular: 10ug |
EUR 380.4 |
Description: Thrombopoietin Human Recombinant produced in E.Coli is a single, non-glycosylated soluble polypeptide chain containing 175 amino acids and having a molecular mass of 18.8kDa which comprises the receptor binding domain of the Mpl-ligand protein.;The TPO is purified by proprietary chromatographic techniques. |
Recombinant Human Thrombopoietin/TPO (N, C-6His) |
CJ95-10ug |
Novoprotein |
10ug |
EUR 242.4 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH 8.0. |
Recombinant Human Thrombopoietin/TPO (N, C-6His) |
CJ95-1mg |
Novoprotein |
1mg |
EUR 2739.6 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH 8.0. |
Recombinant Human Thrombopoietin/TPO (N, C-6His) |
CJ95-500ug |
Novoprotein |
500ug |
EUR 1935.6 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH 8.0. |
Recombinant Human Thrombopoietin/TPO (N, C-6His) |
CJ95-50ug |
Novoprotein |
50ug |
EUR 595.2 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH 8.0. |
Recombinant Human Thrombopoietin/TPO (N, C-6His) |
AP76129 |
SAB |
1mg |
EUR 3489 |
|
Recombinant Human TPO/Thrombopoietin/THPO Protein |
RP00454 |
Abclonal |
10 μg |
EUR 265.2 |
TPO Human, Thrombopoietin Human Recombinant Protein, HEK |
PROTP40225 |
BosterBio |
Regular: 10ug |
EUR 380.4 |
Description: Thrombopoietin Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight range of 80-85kDa due to glycosylation.;The TPO is purified by proprietary chromatographic techniques. |
TPO Thrombopoietin Mouse Recombinant Protein |
PROTP40226 |
BosterBio |
Regular: 10ug |
EUR 380.4 |
Description: Thrombopoietin Mouse Recombinant produced in E.Coli is a single, non-glycosylated soluble polypeptide chain containing 174 amino acids and having a molecular mass of 18704 Dalton._x000D_ The TPO is purified by proprietary chromatographic techniques. _x000D_ |
Recombinant Human Thrombopoietin/TPO Protein, His Tag & His Tag |
E40KMH1801 |
EnoGene |
20ug |
EUR 495 |
Description: Recombinant Human Cytotoxic T-lymphocyte Protein 4 is produced by our Mammalian expression system and the target gene encoding Lys36-Asp161 is expressed with a Flag tag at the C-terminus. |
Recombinant Mouse Thrombopoietin/THPO/TPO (C-6His) |
CS59-10ug |
Novoprotein |
10ug |
EUR 242.4 |
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
Recombinant Mouse Thrombopoietin/THPO/TPO (C-6His) |
CS59-1mg |
Novoprotein |
1mg |
EUR 2983.2 |
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
Recombinant Mouse Thrombopoietin/THPO/TPO (C-6His) |
CS59-500ug |
Novoprotein |
500ug |
EUR 1935.6 |
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
Recombinant Mouse Thrombopoietin/THPO/TPO (C-6His) |
CS59-50ug |
Novoprotein |
50ug |
EUR 595.2 |
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
Recombinant Mouse Thrombopoietin/THPO/TPO (N-6His) |
CP40-10ug |
Novoprotein |
10ug |
EUR 242.4 |
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
Recombinant Mouse Thrombopoietin/THPO/TPO (N-6His) |
CP40-1mg |
Novoprotein |
1mg |
EUR 2983.2 |
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
Recombinant Mouse Thrombopoietin/THPO/TPO (N-6His) |
CP40-500ug |
Novoprotein |
500ug |
EUR 2106 |
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
Recombinant Mouse Thrombopoietin/THPO/TPO (N-6His) |
CP40-50ug |
Novoprotein |
50ug |
EUR 595.2 |
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
Recombinant Mouse Thrombopoietin/THPO/TPO (N-6His) |
AP76322 |
SAB |
1mg |
EUR 3489 |
|
Recombinant Mouse Thrombopoietin/THPO/TPO (C-6His) |
AP76426 |
SAB |
1mg |
EUR 3489 |
|
Recombinant Mouse Thrombopoietin/Tpo/Thpo Protein, His Tag |
E40MOP2291 |
EnoGene |
20ug |
EUR 495 |
Recombinant Human Thrombopoietin |
7-01687 |
CHI Scientific |
2µg |
Ask for price |
Recombinant Human Thrombopoietin |
7-01688 |
CHI Scientific |
10µg |
Ask for price |
Recombinant Human Thrombopoietin |
7-01689 |
CHI Scientific |
1mg |
Ask for price |
Thrombopoietin (TPO) (Human) |
HCOPP-2005 |
Cyagen |
2ug |
EUR 383.4 |
Human Thrombopoietin (Recombinant) |
22060495-1 |
Bio-WORLD |
2 µg |
EUR 146.42 |
Recombinant Human Thrombopoietin, CHO |
7-01690 |
CHI Scientific |
2µg |
Ask for price |
Recombinant Human Thrombopoietin, CHO |
7-01691 |
CHI Scientific |
10µg |
Ask for price |
Recombinant Human Thrombopoietin, CHO |
7-01692 |
CHI Scientific |
1mg |
Ask for price |
Thrombopoietin/TPO |
E21-J95 |
EnoGene |
10ug |
EUR 411.6 |
Recombinant Thrombopoietin Receptor (TPOR) |
RPB468Hu01 |
Cloud-Clone |
10ug |
EUR 191 |
Recombinant Thrombopoietin Receptor (TPOR) |
RPB468Mu01 |
Cloud-Clone |
10ug |
EUR 199 |
Thrombopoietin, Human CHO (TPO) |
22061205-1 |
Bio-WORLD |
2 µg |
EUR 137.27 |
Recombinant Human Thrombopoietin (THPO) (Active) |
CSB-AP003971HU |
Cusabio |
8594 mg |
Ask for price |
Recombinant Human Thrombopoietin/THPO Protein |
RP00174 |
Abclonal |
10 μg |
EUR 219.6 |
Recombinant Human Thrombopoietin (THPO), partial |
CSB-EP023509HU |
Cusabio |
2338 mg |
Ask for price |
Recombinant Human Thrombopoietin(THPO),partial |
AP77785 |
SAB |
1mg |
EUR 1978 |
|
Human Thrombopoietin (TPO)CLIA Kit |
SCA135Hu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 6777.36 |
|
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Thrombopoietin (TPO) in serum, plasma and other biological fluids. |
Human Thrombopoietin (TPO)CLIA Kit |
SCA135Hu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 663.31 |
|
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Thrombopoietin (TPO) in serum, plasma and other biological fluids. |
Human Thrombopoietin (TPO)CLIA Kit |
SCA135Hu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 896.16 |
|
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Thrombopoietin (TPO) in serum, plasma and other biological fluids. |
Human Thrombopoietin (TPO)CLIA Kit |
SCA135Hu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 3672.72 |
|
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Thrombopoietin (TPO) in serum, plasma and other biological fluids. |
Human Thrombopoietin (TPO) CLIA Kit |
4-SCA135Hu |
Cloud-Clone |
-
EUR 6837.60
-
EUR 3673.20
-
EUR 896.40
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Thrombopoietin (TPO)Serum, plasma and other biological fluids |
Human Thrombopoietin,TPO ELISA kit |
201-12-1032 |
SunredBio |
96 tests |
EUR 528 |
|
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Human Thrombopoietin,TPO ELISA kit |
CN-03275H1 |
ChemNorm |
96T |
EUR 642 |
Human Thrombopoietin,TPO ELISA kit |
CN-03275H2 |
ChemNorm |
48T |
EUR 434.4 |
Human Thrombopoietin (TPO) ELISA Kit |
DLR-TPO-Hu-48T |
DL Develop |
48T |
EUR 574.8 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Thrombopoietin (TPO) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Thrombopoietin (TPO) ELISA Kit |
DLR-TPO-Hu-96T |
DL Develop |
96T |
EUR 745.2 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Thrombopoietin (TPO) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Thrombopoietin, TPO ELISA kit |
CSB-E04745h-24T |
Cusabio |
1 plate of 24 wells |
EUR 198 |
|
Description: Quantitativesandwich ELISA kit for measuring Human Thrombopoietin, TPO in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Human Thrombopoietin, TPO ELISA kit |
1-CSB-E04745h |
Cusabio |
-
EUR 843.60
-
EUR 5811.60
-
EUR 3084.00
|
- 1 plate of 96 wells
- 10 plates of 96 wells each
- 5 plates of 96 wells each
|
|
Description: Quantitativesandwich ELISA kit for measuring Human Thrombopoietin, TPO in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
Human Thrombopoietin(TPO)ELISA Kit |
GA-E1048HM-48T |
GenAsia Biotech |
48T |
EUR 346.8 |
Human Thrombopoietin(TPO)ELISA Kit |
GA-E1048HM-96T |
GenAsia Biotech |
96T |
EUR 559.2 |
Human Thrombopoietin,TPO ELISA Kit |
SL1686Hu |
Sunlong |
96 Tests |
EUR 468 |
|
Human Thrombopoietin (TPO) ELISA Kit |
NSL1620Hu |
Sunlong |
96 wells |
EUR 468 |
|
Human Thrombopoietin (TPO) ELISA Kit |
RDR-TPO-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 600 |
Human Thrombopoietin (TPO) ELISA Kit |
RDR-TPO-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 830.4 |
Human Thrombopoietin (TPO) ELISA Kit |
RD-TPO-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 573.6 |
Human Thrombopoietin (TPO) ELISA Kit |
RD-TPO-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 794.4 |
Human Thrombopoietin/Tpo ELISA Kit |
RK00145 |
Abclonal |
96 Tests |
EUR 625.2 |
Human Thrombopoietin (TPO) ELISA Kit |
RD-TPO-Hu-48T |
Reddot Biotech |
48T |
EUR 411.8 |
|
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. |
Human Thrombopoietin (TPO) ELISA Kit |
RD-TPO-Hu-96T |
Reddot Biotech |
96T |
EUR 588.3 |
|
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. |
Human Thrombopoietin (TPO) ELISA Kit |
DLR-TPO-Hu |
DL Develop |
96T |
EUR 422 |
|
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Thrombopoietin (TPO) ELISA Kit |
DL-TPO-Hu |
DL Develop |
96T |
EUR 402 |
|
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Thrombopoietin (TPO) ELISA Kit |
RDR-TPO-Hu-48T |
Reddot Biotech |
48T |
EUR 432.39 |
|
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. |
Human Thrombopoietin (TPO) ELISA Kit |
RDR-TPO-Hu-96T |
Reddot Biotech |
96T |
EUR 617.72 |
|
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. |
Human Thrombopoietin (TPO) ELISA Kit |
SEA135Hu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 5128.02 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Thrombopoietin (TPO) in serum, plasma and other biological fluids. |
Human Thrombopoietin (TPO) ELISA Kit |
SEA135Hu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 527.48 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Thrombopoietin (TPO) in serum, plasma and other biological fluids. |
Human Thrombopoietin (TPO) ELISA Kit |
SEA135Hu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 702.12 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Thrombopoietin (TPO) in serum, plasma and other biological fluids. |
Human Thrombopoietin (TPO) ELISA Kit |
SEA135Hu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 2799.54 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Thrombopoietin (TPO) in serum, plasma and other biological fluids. |
Human Thrombopoietin (TPO) ELISA Kit |
4-SEA135Hu |
Cloud-Clone |
-
EUR 5188.80
-
EUR 2739.60
-
EUR 703.20
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Thrombopoietin (TPO) in samples from Serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species. |
Human Thrombopoietin/TPO ELISA Kit |
E28L0274 |
EnoGene |
96T |
EUR 666.67 |
Human Thrombopoietin(Tpo) ELISA kit |
E22-HC158.48 |
EnoGene |
48T |
EUR 295 |
Human Thrombopoietin(Tpo) ELISA kit |
E22-HC158.96 |
EnoGene |
96T |
EUR 395 |
TPO Human, ThrombopoietinHuman Recombinant Protein, His Tag |
PROTP40225-2 |
BosterBio |
Regular: 10ug |
EUR 380.4 |
Description: TPO produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 340 amino acids (22-353aa.) andhaving a molecular mass of 36.5kDa. (Molecular size on SDS-PAGE will appear atapproximately 40-57KDa).;TPO is expressed with an 8 amino acid His tag at C-Terminusand purified by proprietary chromatographic techniques.. |
Recombinant Human Thrombopoietin R/MPL Protein |
RP02785 |
Abclonal |
500μg |
EUR 279.5 |
|
Active Thrombopoietin (TPO) |
APA135Mu01 |
Cloud-Clone |
10ug |
EUR 277 |
Recombinant Mouse Thrombopoietin |
7-01693 |
CHI Scientific |
2µg |
Ask for price |
Recombinant Mouse Thrombopoietin |
7-01694 |
CHI Scientific |
10µg |
Ask for price |
Recombinant Mouse Thrombopoietin |
7-01695 |
CHI Scientific |
1mg |
Ask for price |
Mouse Thrombopoietin (Recombinant) |
22060602-1 |
Bio-WORLD |
2 µg |
EUR 146.42 |
Recombinant Murine Thrombopoietin |
CC142-002 |
GenDepot |
2ug |
EUR 351.6 |
Recombinant Murine Thrombopoietin |
CC142-010 |
GenDepot |
10ug |
EUR 489.6 |
Recombinant Murine Thrombopoietin |
CC142-101 |
GenDepot |
1mg |
EUR 13344 |
Recombinant Murine Thrombopoietin |
AP60175 |
SAB |
100ug |
EUR 1284 |
|
Thrombopoietin Recombinant Protein |
40-285-0002mg |
ProSci |
0.002 mg |
EUR 311.1 |
Description: TPO is a lineage specific growth factor, produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor and acts as an important regulator of circulating platelets. Human and murine TPO exhibits cross-species reactivity. Recombinant human TPO is a fully biologically active 174 amino acid polypeptide (18.6 kDa), which contains the erythropoietin-like domain of the full length TPO protein. |
Thrombopoietin Recombinant Protein |
40-285-001mg |
ProSci |
0.01 mg |
EUR 437.1 |
Description: TPO is a lineage specific growth factor, produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor and acts as an important regulator of circulating platelets. Human and murine TPO exhibits cross-species reactivity. Recombinant human TPO is a fully biologically active 174 amino acid polypeptide (18.6 kDa), which contains the erythropoietin-like domain of the full length TPO protein. |
Thrombopoietin Recombinant Protein |
92-493 |
ProSci |
0.05 mg |
EUR 821.4 |
Description: Thrombopoietin (TPO) is a glycoprotein hormone which belongs to the EPO/TPO family. It produced by the liver and kidney which regulates the production of platelets. TPO stimulates the production and differentiation of megakaryocytes, the bone marrow cells that bud off large numbers of platelets. Lineage-specific cytokine affects the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. |
Human TPO(Thrombopoietin) ELISA Kit |
EH0422 |
FN Test |
96T |
EUR 571.5 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml |
Human Thrombopoietin ELISA Kit (TPO) |
RK02436 |
Abclonal |
96 Tests |
EUR 625.2 |
Human TPO(Thrombopoietin) ELISA Kit |
ELK0889-48T |
ELK Biotech |
48T |
Ask for price |
|
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TPO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TPO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TPO in the samples is then determined by comparing the OD of the samples to the standard curve. |
Human TPO(Thrombopoietin) ELISA Kit |
ELK0889-96T |
ELK Biotech |
96T |
Ask for price |
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Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TPO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TPO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TPO in the samples is then determined by comparing the OD of the samples to the standard curve. |
Via the engineering of the peptide sequence and duplicate quantity, and by incorporating the fusion lytic gene into the E. coli genome, greater than 99.97% of cells could possibly be lysed inside 30 min of induction no matter cell age. We additional examined the expression and launch of a recombinant enzyme lysostaphin (Lst) and demonstrated that Four h induction of the lytic gene after three h of Lst expression resulted in 98.97% cell lysis. Lst obtained from this method had the identical yield, but 1.63-fold larger exercise, in contrast with that obtained from cells lysed by freeze-thawing and sonication. This autolytic platform reveals potential to be used in large-scale microbial manufacturing of proteins and different biopolymers.