Fucoidan oligosaccharides possesses numerous physicochemical and organic actions. Particular glycoside hydrolases are beneficial instruments for degrading polysaccharides to supply oligosaccharides. On this examine, BcFucA, a novel fucosidase belonging to GH95 household from Bacillus cereus 2-8, was cloned into pET21a vector, expressed in E. coli BL21 (DE3) and characterised. The protein consists of 1136 amino acid residues encoded by 3411 bases and has a molecular weight of 125.35 kDa. The optimum temperature and pH of this enzyme are 50°C and pH 4.0. As well as, this examine confirmed that the unknown perform area (encoding Lys261-Thr681) outlined as a linker is sort of necessary for its exercise. The obtained novel enzyme BcFucA will contribute to the efficient degradation of fucoidan and future industrial purposes.
Optimization of medium composition to extend the expression of <em>recombinant</em> human interferon-β utilizing the Plackett-Burman and central composite design in <i><em>E</em>. <em>coli</em></i> S<em>E</em>1
Recombinant human interferon-β (rhIFN-β) is therapeutically necessary and new commercially viable approaches are wanted for its elevated manufacturing. On this examine, a codon-optimized gene encoding for rhIFN-β(C17S) protein was designed and expressed in E. coli SE1. As a primary step of medium optimization, progress of E. coli as a perform of various media parts was studied. Subsequently, to optimize the media composition, a response floor methodology (RSM) was used.
Our outcomes present that optimized medium (15.Zero g/L tryptone, 12.three g/L meat extract, 1.Zero g/L MgSO4 and 0.5 g/L thiamine together with minimal medium) obtained on this examine present higher progress of recombinant cells and the expression degree of recombinant protein was ~ 1.7-fold greater than Luria-Bertani medium. The optimized medium could also be utilized for the large-scale manufacturing of rhIFN-β.
Purposeful analysis of a recombinant fungal immunomodulatory protein from L. rhinocerus produced in P. pastoris and E. coli host expression techniques
Fungal immunomodulatory proteins (FIPs) are bioactive proteins with immunomodulatory properties. We beforehand reported the heterologous manufacturing in Escherichia coli of FIP-Lrh from Tiger milk mushroom (Lignosus rhinocerus) with potent cytotoxic impact on most cancers cell strains. Nonetheless, protein produced in E. coli lacks post-translational modifications and could also be contaminated with lipopolysaccharide (LPS) endotoxin. Due to this fact, on this examine, yFIP-Lrh produced in Pichia pastoris was functionally in contrast with eFIP-Lrh produced in E. coli.
Expression assemble of FIP-Lrh cDNA in pPICZα was generated, reworked into P. pastoris X-33 and Mut+ transformants have been verified by colony PCR. Induction with 0.5% or 1% methanol resulted in a secreted 13.6 kDa yFIP-Lrh which was subsequently purified and verified utilizing LCMS/MS evaluation. Measurement exclusion chromatography confirmed eFIP-Lrh as a homodimer whereas the bigger measurement of yFIP-Lrh could point out post-translational modification regardless of damaging for glycoproteins staining.
At decrease focus (4-Eight μg/mL), yFIP-Lrh induced considerably larger Th1 (IFN-γ, TNF-α) and Th2 (IL-6, IL-4, IL-5, IL-13) cytokines manufacturing in mice splenocytes, whereas 16 μg/mL eFIP-Lrh induced considerably larger pro-inflammatory cytokines (TNF-α, IL-6, IL-10), probably attributable to larger residual LPS endotoxin (0.082 EU/mL) in eFIP-Lrh in comparison with negligible degree in yFIP-Lrh (0.001 EU/mL).
Moreover, yFIP-Lrh confirmed larger cytotoxic impact on MCF-7 and HeLa most cancers cells. Since each recombinant proteins of FIP-Lrh have the identical peptide sequence, moreover glycosylation, different post-translational modifications in yFIP-Lrh could account for its enhanced immunomodulatory and anti-proliferative actions.
In conclusion, P. pastoris is most popular over E. coli for manufacturing of a functionally energetic yFIP-Lrh devoid of endotoxin contamination. KEY POINTS: • FIP-Lrh can induced manufacturing of Th1 and Th2 cytokines by mouse splenocytes. • Larger cytotoxic impact on most cancers cells noticed for yeast in comparison with E. coli produced FIP-Lrh. • P. pastoris permits manufacturing of an endotoxin-free and functionally energetic recombinant FIP-Lrh.
Endolysin-Primarily based Autolytic E. coli System for Facile Restoration of Recombinant Proteins
Restoration of recombinant proteins from the Escherichia coli cytoplasm will depend on cell disruption by mechanical, chemical, and/or enzymatic strategies, which normally trigger incomplete cell breakage or protein denaturation. Controllable autolytic E. coli strains have been designed to facilitate the purification of recombinant proteins; nevertheless, these strains undergo from low restoration yield, sluggish cell lysis, or in depth pressure engineering. Herein, we report an improved, extremely environment friendly programmable autolytic E. coli platform, through which cell lysis is initiated upon the induced expression of T4 lysozyme with N-terminal fusion of a cell-penetrating peptide.
) Recombinant Thrombopoietin (TPO) |
|
MBS2139838-05mg |
MyBiosource |
0.5mg |
EUR 1180 |
Recombinant Thrombopoietin (TPO) |
|
MBS2031758-001mg |
MyBiosource |
0.01mg |
EUR 165 |
Recombinant Thrombopoietin (TPO) |
|
MBS2031758-005mg |
MyBiosource |
0.05mg |
EUR 290 |
Recombinant Thrombopoietin (TPO) |
|
MBS2031758-01mg |
MyBiosource |
0.1mg |
EUR 435 |
Recombinant Thrombopoietin (TPO) |
|
MBS2031758-02mg |
MyBiosource |
0.2mg |
EUR 525 |
Recombinant Thrombopoietin (TPO) |
|
MBS2031758-05mg |
MyBiosource |
0.5mg |
EUR 1005 |
Recombinant Thrombopoietin (TPO) |
|
MBS2029261-001mg |
MyBiosource |
0.01mg |
EUR 170 |
Recombinant Thrombopoietin (TPO) |
|
MBS2029261-005mg |
MyBiosource |
0.05mg |
EUR 305 |
Recombinant Thrombopoietin (TPO) |
|
MBS2029261-01mg |
MyBiosource |
0.1mg |
EUR 460 |
Recombinant Thrombopoietin (TPO) |
|
MBS2029261-02mg |
MyBiosource |
0.2mg |
EUR 560 |
Recombinant Thrombopoietin (TPO) |
|
MBS2029261-05mg |
MyBiosource |
0.5mg |
EUR 1065 |
Recombinant Thrombopoietin (TPO) |
|
MBS2122575-001mg |
MyBiosource |
0.01mg |
EUR 170 |
Recombinant Thrombopoietin (TPO) |
|
MBS2122575-005mg |
MyBiosource |
0.05mg |
EUR 305 |
Recombinant Thrombopoietin (TPO) |
|
MBS2122575-01mg |
MyBiosource |
0.1mg |
EUR 460 |
Recombinant Thrombopoietin (TPO) |
|
MBS2122575-02mg |
MyBiosource |
0.2mg |
EUR 560 |
Recombinant Thrombopoietin (TPO) |
|
MBS2122575-05mg |
MyBiosource |
0.5mg |
EUR 1065 |
Recombinant Thrombopoietin (TPO) |
|
MBS2029986-001mg |
MyBiosource |
0.01mg |
EUR 165 |
Recombinant Thrombopoietin (TPO) |
|
MBS2029986-005mg |
MyBiosource |
0.05mg |
EUR 285 |
Recombinant Thrombopoietin (TPO) |
|
MBS2029986-01mg |
MyBiosource |
0.1mg |
EUR 425 |
Recombinant Thrombopoietin (TPO) |
|
MBS2029986-02mg |
MyBiosource |
0.2mg |
EUR 515 |
Recombinant Thrombopoietin (TPO) |
|
MBS2029986-05mg |
MyBiosource |
0.5mg |
EUR 980 |
Recombinant Thrombopoietin (TPO) |
|
MBS2097683-001mg |
MyBiosource |
0.01mg |
EUR 155 |
Recombinant Thrombopoietin (TPO) |
|
MBS2097683-005mg |
MyBiosource |
0.05mg |
EUR 260 |
Recombinant Thrombopoietin (TPO) |
|
MBS2097683-01mg |
MyBiosource |
0.1mg |
EUR 375 |
Recombinant Thrombopoietin (TPO) |
|
MBS2097683-02mg |
MyBiosource |
0.2mg |
EUR 460 |
Recombinant Thrombopoietin (TPO) |
|
MBS2097683-05mg |
MyBiosource |
0.5mg |
EUR 860 |
Recombinant Thrombopoietin (TPO) |
|
RPA135Ca01 |
Cloud-Clone |
10ug |
EUR 196 |
Recombinant Thrombopoietin (TPO) |
|
RPA135Ga01 |
Cloud-Clone |
10ug |
EUR 176 |
Recombinant Thrombopoietin (TPO) |
|
RPA135Hu01 |
Cloud-Clone |
10ug |
EUR 164 |
Recombinant Thrombopoietin (TPO) |
|
4-RPA135Hu01 |
Cloud-Clone |
-
Ask for price
-
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-
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-
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-
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-
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-
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- 10ug
- 50ug
- 100 ug
- 200 ug
- 500 ug
- 1 mg
- 5 mg
|
|
|
|
Description: Recombinant Human Thrombopoietin expressed in: E.coli |
Recombinant Thrombopoietin (TPO) |
|
RPA135Hu02 |
Cloud-Clone |
10ug |
EUR 160 |
Recombinant Thrombopoietin (TPO) |
|
4-RPA135Hu02 |
Cloud-Clone |
-
Ask for price
-
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-
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-
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-
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-
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-
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- 10ug
- 50ug
- 100 ug
- 200 ug
- 500 ug
- 1 mg
- 5 mg
|
|
|
|
Description: Recombinant Human Thrombopoietin expressed in: E.coli |
Recombinant Thrombopoietin (TPO) |
|
RPA135Hu03 |
Cloud-Clone |
10ug |
EUR 166 |
Recombinant Thrombopoietin (TPO) |
|
RPA135Hu04 |
Cloud-Clone |
10ug |
EUR 165 |
Recombinant Thrombopoietin (TPO) |
|
RPA135Mu01 |
Cloud-Clone |
10ug |
EUR 140 |
Recombinant Thrombopoietin (TPO) |
|
4-RPA135Mu01 |
Cloud-Clone |
-
Ask for price
-
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-
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-
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-
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-
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-
Ask for price
|
- 10ug
- 50ug
- 100 ug
- 200 ug
- 500 ug
- 1 mg
- 5 mg
|
|
|
|
Description: Recombinant Mouse Thrombopoietin expressed in: E.coli |
Recombinant Thrombopoietin (TPO) |
|
RPA135Po01 |
Cloud-Clone |
10ug |
EUR 164 |
Recombinant Thrombopoietin (TPO) |
|
RPA135Po02 |
Cloud-Clone |
10ug |
EUR 166 |
Recombinant Thrombopoietin (TPO) |
|
RPA135Po03 |
Cloud-Clone |
10ug |
EUR 166 |
Recombinant Thrombopoietin (TPO) |
|
RPA135Ra01 |
Cloud-Clone |
10ug |
EUR 176 |
Recombinant Thrombopoietin (TPO) |
|
4-RPA135Ra01 |
Cloud-Clone |
-
Ask for price
-
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-
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-
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-
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-
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-
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|
- 10ug
- 50ug
- 100 ug
- 200 ug
- 500 ug
- 1 mg
- 5 mg
|
|
|
|
Description: Recombinant Rat Thrombopoietin expressed in: E.coli |
 TPO Thrombopoietin Human Recombinant Protein |
|
PROTP40225-1 |
BosterBio |
Regular: 10ug |
EUR 380.4 |
|
Description: Thrombopoietin Human Recombinant produced in E.Coli is a single, non-glycosylated soluble polypeptide chain containing 175 amino acids and having a molecular mass of 18.8kDa which comprises the receptor binding domain of the Mpl-ligand protein.;The TPO is purified by proprietary chromatographic techniques. |
) Recombinant Human Thrombopoietin/TPO (N, C-6His) |
|
CJ95-10ug |
Novoprotein |
10ug |
EUR 242.4 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH 8.0. |
Recombinant Human Thrombopoietin/TPO (N, C-6His) |
|
CJ95-1mg |
Novoprotein |
1mg |
EUR 2739.6 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH 8.0. |
Recombinant Human Thrombopoietin/TPO (N, C-6His) |
|
CJ95-500ug |
Novoprotein |
500ug |
EUR 1935.6 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH 8.0. |
Recombinant Human Thrombopoietin/TPO (N, C-6His) |
|
CJ95-50ug |
Novoprotein |
50ug |
EUR 595.2 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH 8.0. |
Recombinant Human Thrombopoietin/TPO (N, C-6His) |
|
AP76129 |
SAB |
1mg |
EUR 3489 |
|
|
 Recombinant Human TPO/Thrombopoietin/THPO Protein |
|
RP00454 |
Abclonal |
10 μg |
EUR 240.89 |
 TPO Human, Thrombopoietin Human Recombinant Protein, HEK |
|
PROTP40225 |
BosterBio |
Regular: 10ug |
EUR 380.4 |
|
Description: Thrombopoietin Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight range of 80-85kDa due to glycosylation.;The TPO is purified by proprietary chromatographic techniques. |
) Recombinant Human Thrombopoietin/TPO Protein (N, C-6His) |
|
MBS2553281-001mg |
MyBiosource |
0.01mg |
EUR 220 |
Recombinant Human Thrombopoietin/TPO Protein (N, C-6His) |
|
MBS2553281-005mg |
MyBiosource |
0.05mg |
EUR 505 |
Recombinant Human Thrombopoietin/TPO Protein (N, C-6His) |
|
MBS2553281-5x005mg |
MyBiosource |
5x0.05mg |
EUR 2270 |
 (Human)) Thrombopoietin (TPO) (Human) |
|
HCOPP-2005 |
Cyagen |
2ug |
EUR 383.4 |
 Human) Thrombopoietin (TPO) Human |
|
GWB-598980 |
GenWay Biotech |
0.01 mg |
Ask for price |
) Thrombopoietin, Recombinant, Human (TPO, Mpl-ligand, MGDF) |
|
MBS650901-0002mg |
MyBiosource |
0.002mg |
EUR 375 |
Thrombopoietin, Recombinant, Human (TPO, Mpl-ligand, MGDF) |
|
MBS650901-001mg |
MyBiosource |
0.01mg |
EUR 455 |
Thrombopoietin, Recombinant, Human (TPO, Mpl-ligand, MGDF) |
|
MBS650901-5x001mg |
MyBiosource |
5x0.01mg |
EUR 1890 |
(Active)) Recombinant Human Thrombopoietin/TPO Protein (His Tag)(Active) |
|
PKSH033110-10ug |
Elabscience Biotech |
10ug |
EUR 178 |
|
|
|
Description: Human |
Recombinant Human Thrombopoietin/TPO Protein (His Tag)(Active) |
|
PKSH033110-50ug |
Elabscience Biotech |
50ug |
EUR 523 |
|
|
|
Description: Human |
 Recombinant Human Thrombopoietin/TPO Protein, His Tag & His Tag |
|
E40KMH1801 |
EnoGene |
20ug |
EUR 495 |
|
Description: Recombinant Human Cytotoxic T-lymphocyte Protein 4 is produced by our Mammalian expression system and the target gene encoding Lys36-Asp161 is expressed with a Flag tag at the C-terminus. |
) Thrombopoietin, Human CHO (TPO) |
|
22061205-1 |
Bio-WORLD |
2 µg |
EUR 176.77 |
 Thrombopoietin/TPO |
|
E21-J95 |
EnoGene |
10ug |
EUR 225 |
|
|
|
Description: Recombinant Human Thrombopoietin is produced by our Mammalian expression system and the target gene encoding Ser22-Gly353 is expressed with a 6His tag at the N-terminus, 6His tag at the C-terminus. |
Thrombopoietin/TPO |
|
MBS8532998-001mg |
MyBiosource |
0.01mg |
EUR 305 |
Thrombopoietin/TPO |
|
MBS8532998-5x001mg |
MyBiosource |
5x0.01mg |
EUR 1220 |
 Recombinant Human Thrombopoietin/TPO protein, N-His Tag & C-His Tag |
|
MBS1560959-005mg |
MyBiosource |
0.05mg |
EUR 345 |
Recombinant Human Thrombopoietin/TPO protein, N-His Tag & C-His Tag |
|
MBS1560959-01mg |
MyBiosource |
0.1mg |
EUR 450 |
Recombinant Human Thrombopoietin/TPO protein, N-His Tag & C-His Tag |
|
MBS1560959-5x01mg |
MyBiosource |
5x0.1mg |
EUR 1710 |
) Recombinant Mouse Thrombopoietin/TPO Protein (His Tag) |
|
PKSM041156-10ug |
Elabscience Biotech |
10ug |
EUR 178 |
|
|
|
Description: Mouse |
Recombinant Mouse Thrombopoietin/TPO Protein (His Tag) |
|
PKSM041156-50ug |
Elabscience Biotech |
50ug |
EUR 523 |
|
|
|
Description: Mouse |
 Recombinant Mouse TPO/Thrombopoietin/THPO Protein |
|
RP00777 |
Abclonal |
500μg |
EUR 286.94 |
 CLIA Kit) Human Thrombopoietin (TPO) CLIA Kit |
|
MBS2025315-10x96StripWells |
MyBiosource |
10x96-Strip-Wells |
EUR 6205 |
Human Thrombopoietin (TPO) CLIA Kit |
|
MBS2025315-24StripWells |
MyBiosource |
24-Strip-Wells |
EUR 405 |
Human Thrombopoietin (TPO) CLIA Kit |
|
MBS2025315-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 625 |
Human Thrombopoietin (TPO) CLIA Kit |
|
MBS2025315-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 3400 |
Human Thrombopoietin (TPO) CLIA Kit |
|
MBS2025315-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 845 |
Human Thrombopoietin (TPO) CLIA Kit |
|
MBS2708713-10x96StripWells |
MyBiosource |
10x96-Strip-Wells |
EUR 3735 |
Human Thrombopoietin (TPO) CLIA Kit |
|
MBS2708713-24StripWells |
MyBiosource |
24-Strip-Wells |
EUR 275 |
Human Thrombopoietin (TPO) CLIA Kit |
|
MBS2708713-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 365 |
Human Thrombopoietin (TPO) CLIA Kit |
|
MBS2708713-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 1935 |
Human Thrombopoietin (TPO) CLIA Kit |
|
MBS2708713-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 480 |
CLIA Kit) Human Thrombopoietin (TPO)CLIA Kit |
|
SCA135Hu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 6777.36 |
|
|
|
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Thrombopoietin (TPO) in serum, plasma and other biological fluids. |
Human Thrombopoietin (TPO)CLIA Kit |
|
SCA135Hu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 663.31 |
|
|
|
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Thrombopoietin (TPO) in serum, plasma and other biological fluids. |
Human Thrombopoietin (TPO)CLIA Kit |
|
SCA135Hu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 896.16 |
|
|
|
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Thrombopoietin (TPO) in serum, plasma and other biological fluids. |
Human Thrombopoietin (TPO)CLIA Kit |
|
SCA135Hu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 3672.72 |
|
|
|
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Thrombopoietin (TPO) in serum, plasma and other biological fluids. |
Human Thrombopoietin (TPO) CLIA Kit |
|
4-SCA135Hu |
Cloud-Clone |
-
Ask for price
-
Ask for price
-
Ask for price
|
- 1 plate of 96 wells
- 5 plates of 96 wells
- 10 plates of 96 wells
|
|
|
|
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Thrombopoietin (TPO)Serum, plasma and other biological fluids |
 Human Thrombopoietin,TPO ELISA kit |
|
201-12-1032 |
Shanghai Sunred Biological Technology |
96 tests |
EUR 528 |
|
|
|
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
 Human Thrombopoietin, TPO ELISA kit |
|
CSB-E04745h-24T |
Cusabio |
1 plate of 24 wells |
EUR 198 |
|
|
|
Description: Quantitativesandwich ELISA kit for measuring Human Thrombopoietin, TPO in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Human Thrombopoietin,TPO ELISA kit |
|
CSB-E04745h-48T |
Cusabio |
48T |
EUR 290.06 |
|
|
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Description: Homo sapiens (Human) |
Human Thrombopoietin, TPO ELISA kit |
|
1-CSB-E04745h |
Cusabio |
-
Ask for price
-
Ask for price
-
Ask for price
|
- 5 plates of 96 wells each
- 1 plate of 96 wells
- 10 plates of 96 wells each
|
|
|
|
Description: Quantitativesandwich ELISA kit for measuring Human Thrombopoietin, TPO in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
Human Thrombopoietin,TPO ELISA kit |
|
CSB-E04745h-96T |
Cusabio |
96T |
EUR 446.25 |
|
|
|
Description: Homo sapiens (Human) |
 ELISA Kit) Human Thrombopoietin (TPO) ELISA Kit |
|
DLR-TPO-Hu |
DL Develop |
96T |
EUR 422 |
|
|
|
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Thrombopoietin (TPO) ELISA Kit |
|
DLR-TPO-Hu-48T |
DL Develop |
48T |
EUR 574.8 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Thrombopoietin (TPO) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Thrombopoietin (TPO) ELISA Kit |
|
DLR-TPO-Hu-96T |
DL Develop |
96T |
EUR 745.2 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Thrombopoietin (TPO) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Thrombopoietin (TPO) ELISA Kit |
|
DL-TPO-Hu |
DL Develop |
96T |
EUR 402 |
|
|
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Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Thrombopoietin (TPO) ELISA Kit |
|
AE17355HU-48T |
Abebio |
48T |
EUR 360 |
|
|
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Description: Human (Homo sapiens) |
Human Thrombopoietin (TPO) ELISA Kit |
|
AE17355HU-96T |
Abebio |
96T |
EUR 570 |
|
|
|
Description: Human (Homo sapiens) |
Human Thrombopoietin,TPO ELISA kit |
|
CN-03275H1 |
ChemNorm |
96T |
EUR 642 |
Human Thrombopoietin,TPO ELISA kit |
|
CN-03275H2 |
ChemNorm |
48T |
EUR 434.4 |
ELISA Kit) Human Thrombopoietin(TPO)ELISA Kit |
|
GA-E1048HM-48T |
GenAsia Biotech |
48T |
EUR 346.8 |
Human Thrombopoietin(TPO)ELISA Kit |
|
GA-E1048HM-48Tests |
GenAsia Biotech |
48 Tests |
Ask for price |
Human Thrombopoietin(TPO)ELISA Kit |
|
GA-E1048HM-96T |
GenAsia Biotech |
96T |
EUR 559.2 |
Human Thrombopoietin(TPO)ELISA Kit |
|
GA-E1048HM-96Tests |
GenAsia Biotech |
96 Tests |
EUR 388 |
 Human Thrombopoietin,TPO ELISA KIT |
|
JOT-EK1031Hu |
Jotbody |
96 wells |
Ask for price |
|
Description: Human |
 ELISA kit) Human Thrombopoietin(Tpo) ELISA kit |
|
E22-HC158.48 |
EnoGene |
48T |
EUR 295 |
|
|
|
Description: 31pg/ml |
Human Thrombopoietin(Tpo) ELISA kit |
|
E22-HC158.96 |
EnoGene |
96T |
EUR 395 |
|
|
|
Description: 31pg/ml |
 Human Thrombopoietin/TPO ELISA Kit |
|
E28L0274 |
EnoGene |
96T |
EUR 666.67 |
ELISA kit) Human Thrombopoietin (TPO)ELISA kit |
|
EIA06434h |
Enlibio |
each |
EUR 430 |
Via the engineering of the peptide sequence and duplicate quantity, and by incorporating the fusion lytic gene into the E. coli genome, greater than 99.97% of cells could possibly be lysed inside 30 min of induction no matter cell age. We additional examined the expression and launch of a recombinant enzyme lysostaphin (Lst) and demonstrated that Four h induction of the lytic gene after three h of Lst expression resulted in 98.97% cell lysis. Lst obtained from this method had the identical yield, but 1.63-fold larger exercise, in contrast with that obtained from cells lysed by freeze-thawing and sonication. This autolytic platform reveals potential to be used in large-scale microbial manufacturing of proteins and different biopolymers.
